Antibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Western blot [1]
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Validation data
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- Product number
- AM00058PU-N - Provider product page
- Provider
- OriGene
- Product name
- ERK5 (MAPK7) (incl. pos. control) mouse monoclonal antibody, clone 12F2, Purified
- Antibody type
- Monoclonal
- Description
- ERK5 (MAPK7) (incl. pos. control) mouse monoclonal antibody, clone 12F2, Purified
- Host
- Mouse
- Conjugate
- Unconjugated
- Epitope
- MAPK7
- Isotype
- IgG
- Antibody clone number
- 12F2
- Vial size
- 100 µg
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Supportive validation
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- OriGene (provider)
- Main image
- Experimental details
- Detection of endogenous MAPK7 / erk5 Whole cell lysates of serum starved tumor cells (20.000 cells per lane) were applied to SDS-PAGE and transferred to a PVDF membrane. Immunoblots were probed with mab MAPK7-12F2 (0.5 ug/ ml) for 1h at RT and developed by ECL (exp. time: 30 sec). Lane 1: A431 Lane 2: A549 Lane 3: SKOV3 Lane 4: OVCAR5 Lane 5: HaCaT Lane 6: PC3 Lane 7: HeLa Lane 8: HepG2
- Validation comment
- WB