AM00058PU-N

antibody from OriGene

Targeting: MAPK7 BMK1, ERK5, PRKM7

Western blot (Supportive data in Antibodypedia)

Antibody Data

Product number

AM00058PU-N

Provider

OriGene

Product name

ERK5 (MAPK7) (incl. pos. control) mouse monoclonal antibody, clone 12F2, Purified

Antibody type

Monoclonal

Description

ERK5 (MAPK7) (incl. pos. control) mouse monoclonal antibody, clone 12F2, Purified

Host

Mouse

Conjugate

Unconjugated

Epitope

MAPK7

Isotype

IgG

Antibody clone number

12F2

Vial size

100 µg

Western blot

Supportive validation

Submitted by

OriGene (provider)

Main image

Experimental details

Detection of endogenous MAPK7 / erk5 Whole cell lysates of serum starved tumor cells (20.000 cells per lane) were applied to SDS-PAGE and transferred to a PVDF membrane. Immunoblots were probed with mab MAPK7-12F2 (0.5 ug/ ml) for 1h at RT and developed by ECL (exp. time: 30 sec). Lane 1: A431 Lane 2: A549 Lane 3: SKOV3 Lane 4: OVCAR5 Lane 5: HaCaT Lane 6: PC3 Lane 7: HeLa Lane 8: HepG2

Validation comment

WB