PA5-27483

antibody from Invitrogen Antibodies

Targeting: PPP2CB PP2Abeta

Western blot (Supportive data in Antibodypedia)

Immunocytochemistry (Supportive data in Antibodypedia)

Antibody Data

Product number

PA5-27483

Provider

Invitrogen Antibodies

Product name

PP2A beta Polyclonal Antibody

Antibody type

Polyclonal

Antigen

Recombinant protein fragment

Description

Recommended positive controls: 293T, A431, mouse brain.

Concentration

1 mg/mL

Western blot

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Western Blot analysis of PP2A beta was performed by separating 30 µg of non-transfected (–) and transfected (+) 293T whole cell extracts by 10% SDS-PAGE. Proteins were transferred to a membrane and probed with a PP2A beta Polyclonal Antibody (Product # PA5-27483) at a dilution of 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Western Blot using PP2A beta Polyclonal Antibody (Product # PA5-27483). Sample (30 µg of whole cell lysate). A: 293T. Lane B: A431. 12% SDS PAGE. PP2A beta Polyclonal Antibody (Product # PA5-27483) diluted at 1:500.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Western Blot using PP2A beta Polyclonal Antibody (Product # PA5-27483). Sample (50 µg of whole cell lysate). Lane A: Mouse brain. 10% SDS PAGE. PP2A beta Polyclonal Antibody (Product # PA5-27483) diluted at 1:1,000.

Immunocytochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunofluorescent analysis of PP2A beta in paraformaldehyde-fixed HeLa cells using a PP2A beta polyclonal antibody (Product # PA5-27483) at a 1:500 dilution.