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Flow cytometryAntibody data
- Antibody Data
- Antigen structure
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- Validations
- Flow cytometry [1]
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- Product number
- MAB6940 - Provider product page
- Provider
- Abnova Corporation
- Proper citation
- Abnova Corporation Cat#MAB6940, RRID:AB_10681066
- Product name
- ITGA2B monoclonal antibody, clone 96.2C1
- Antibody type
- Monoclonal
- Description
- Mouse monoclonal antibody raised against native ITGA2B.
- Isotype
- IgG
- Antibody clone number
- 96.2C1
- Storage
- Store at 4°C.
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Supportive validation
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- Abnova Corporation (provider)
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- Experimental details
- 5 X 105 ficoll prepared human peripheral blood lymphocytes were washed and preincubated 5 minutes with 20 uL of 250 ug/mL human IgG (To block non specific binding) after which they were incubated 45 minutes on ice with 80 uL of ITGA2B monoclonal antibody, clone 96.2C1 (Cat # MAB6940) at 10 ug/mL. Cells were washed twice and incubated with Goat anti-Mouse IgG/FITC, after which they were washed three times, fixed and analyzed by FACS. A net 17% sub population of the cells stained positive with a mean shift of 2.5 log10 fluorescent units when compared to a Mouse IgG1 negative control at a similar concentration.
- Validation comment
- Flow Cytometry
