PA5-79527

antibody from Invitrogen Antibodies

Targeting: ITGA2B CD41, CD41B, GP2B, PPP1R93

Western blot

Immunohistochemistry

Antibody data

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Validation data

Product number: PA5-79527 - Provider product page
Provider: Invitrogen Antibodies
Product name: CD41 Polyclonal Antibody
Antibody type: Polyclonal
Antigen: Synthetic peptide
Description: Reconstitute with 0.2 mL of distilled water to yield a concentration of 500 µg/mL.
Reactivity: Human, Mouse, Rat
Host: Rabbit
Isotype: IgG
Vial size: 100 µg
Concentration: 500 µg/mL
Storage: -20°C

ITGA2B protein structure - PA5-79527 shown in red.

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: 2 FIGURE Composition of thrombi formed using the mouse IVC stenosis and stasis models of thrombosis. Thrombus composition was assessed by western blotting of total soluble protein lysates from thrombi formed using the IVC stenosis and IVC stasis models of thrombosis (n = 7 per group). A, Representative western blots for fibrin(ogen), the platelet marker CD41, the neutrophil marker Ly6G, the cytoplasmic marker beta Actin, citrullinated histone H3 (H3Cit), and the nuclear marker total histone H3 (H3) in thrombi formed using the IVC stenosis and IVC stasis models. Densitometric analysis of (B) fibrin(ogen), (C) CD41, (D) Ly6G, (E) beta Actin, (F) H3Cit, and (G) H3 in thrombi formed in the IVC stenosis model compared with the IVC stasis model. H, The ratio of H3Cit to total H3 was significantly higher in thrombi formed in the IVC stenosis model compared with the IVC stasis model. * P < .05, ** P < .01 Mann-Whitney U test. Data represented as individual values with a line for the median

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: 3 FIGURE Composition of thrombi formed using the IVC stasis is F12 +/+ mice compared with F12 -/- mice and F11 +/+ mice compared with F11 -/- mice. Thrombus composition was assessed in thrombi from F12 -/- and F11 -/- mice formed using the IVC stasis model of thrombosis by western blotting of total soluble protein lysates and compared with respective wild-type littermate controls. Densitometric analysis of immunoblots for (A, H) fibrin(ogen), (B, I) CD41, (C, J) Ly6G, (D, K) beta actin, (E, L) citrullinated histone H3, and (F, M) total histone H3 for F12 -/- , F11 -/- mice and wild-type littermate controls. (G, N) The ratio of H3Cit to total H3 was also calculated. * P < .01 Mann-Whitney U test. Data represented as individual values plus the median

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Figure 3. Sequestration of platelets in PEAs corresponds with platelet decline during acute mCMV infection. (A) Representative immunohistochemistry images (400x) of CD41+ platelets (brown, indicated by black arrowhead) bound to CD34+ vessels (red) in the salivary gland of a mCMV-infected mouse. (B) Quantification of PEA formation in the salivary gland longitudinally throughout acute mCMV infection. Kruskal-Wallis analyses with Dunn's posttest, excluding outlier as determined by Grubs outlier test, bars indicate median values.