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Learn37-3800
antibody from Invitrogen Antibodies
Targeting: LRP1
A2MR, APOER, APR, CD91, IGFBP-3R, IGFBP3R1, LRP, LRP1A
Western blot
ImmunocytochemistryAntibody data
- Antibody Data
- Antigen structure
- References [3]
- Comments [0]
- Validations
- Immunocytochemistry [1]
- Immunohistochemistry [1]
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- Product number
- 37-3800 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- LRP1 Monoclonal Antibody (A2MR-alpha-2)
- Antibody type
- Monoclonal
- Antigen
- Purifed from natural sources
- Description
- 37-3800 has been successfully used in immunohistochemistry analysis of LRP1 in mouse E16.5 frozen lung sections.
- Reactivity
- Human, Mouse
- Host
- Mouse
- Isotype
- IgG
- Antibody clone number
- A2MR-alpha-2
- Vial size
- 100 µg
- Concentration
- 0.5 mg/mL
- Storage
- -20°C
Submitted references The significance of heat-shock protein gp96 and its receptors' CD91 and Toll-like receptor 4 expression at the maternal foetal interface.
Distribution of the alpha 2-macroglobulin receptor/low density lipoprotein receptor-related protein in human tissues.
Distribution of the alpha 2-macroglobulin receptor/low density lipoprotein receptor-related protein in human tissues.
Gulic T, Laskarin G, Redzovic A, Eminović S, Haller H, Rukavina D
American journal of reproductive immunology (New York, N.Y. : 1989) 2013 Jul;70(1):10-23
American journal of reproductive immunology (New York, N.Y. : 1989) 2013 Jul;70(1):10-23
Distribution of the alpha 2-macroglobulin receptor/low density lipoprotein receptor-related protein in human tissues.
Moestrup SK, Gliemann J, Pallesen G
Cell and tissue research 1992 Sep;269(3):375-82
Cell and tissue research 1992 Sep;269(3):375-82
Distribution of the alpha 2-macroglobulin receptor/low density lipoprotein receptor-related protein in human tissues.
Moestrup SK, Gliemann J, Pallesen G
Cell and tissue research 1992 Sep;269(3):375-82
Cell and tissue research 1992 Sep;269(3):375-82
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunofluorescence analysis of Prolow-density lipoprotein receptor-related protein 1 was performed using 70% confluent log phase U-87 MG and SK-BR-3 cells. The cells were fixed with 4% paraformaldehyde for 5 minutes, permeabilized with 0.1% Triton™ X-100 for 10 minutes, and blocked with 2% BSA for overnight at room temperature. The cells were labeled with LRP1 Monoclonal Antibody (A2MR-alpha-2) (Product # 37-3800) at 1:100 dilution in 0.1% BSA, incubated at 4 degree celsius overnight and then labeled with Donkey anti-Mouse IgG (H+L) Highly Cross-Adsorbed Secondary Antibody, Alexa Fluor Plus 647 (Product # A32787), (1:2000 dilution), for 45 minutes at room temperature (Panel a: Green). Nuclei (Panel b:Blue) were stained with ProLong™ Diamond Antifade Mountant with DAPI (Product # P36962). F-actin (Panel c: Red) was stained with Rhodamine Phalloidin (Product # R415, 1:300). Panel d represents the merged image showing Golgi Body localization. Panel (e-h) represents SK-BR-3 cells showing no expression of LRP1. The images were captured at 60x magnification.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemistry analysis of LRP1 in 4% PFA, OCT embedded frozen mouse E16.5 lung sections. Sections were blocked and probed with a LRP1 mouse monoclonal antibody (Product # 37-3800) at a dilution of 1:100. Detection was performed using an Alexa Fluor-568-conjugated anti-mouse secondary antibody (Product # A-11004).
