37-7600

antibody from Invitrogen Antibodies

Targeting: LRP1 A2MR, APOER, APR, CD91, IGFBP-3R, IGFBP3R1, LRP, LRP1A

Western blot (Recommended by provider)

ELISA (Recommended by provider)

Immunocytochemistry (Supportive data in Antibodypedia)

Immunohistochemistry (Data presented on provider website)

Flow cytometry (Recommended by provider)

Other assay (Supportive data in Antibodypedia)

Antibody Data

Product number

37-7600

Provider

Invitrogen Antibodies

Product name

LRP1 Monoclonal Antibody (A2MR-beta-1)

Antibody type

Monoclonal

Antigen

Synthetic peptide

Description

37-7600 was used in IHC to successfully detect LRP-1 in mouse E18.5 lung section.

Reactivity

Human, Mouse, Rat

Host

Mouse

Isotype

IgG

Antibody clone number

A2MR-beta-1

Vial size

100 µg

Concentration

0.5 mg/mL

Storage

-20°C

References

Submitted references

LRP-1 receptor combines EGFR signalling and eHsp90α autocrine to support constitutive breast cancer cell motility in absence of blood supply.

Chang C, Tang X, Mosallaei D, Chen M, Woodley DT, Schönthal AH, Li W
Scientific reports 2022 Jul 14;12(1):12006

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Human neural cell type-specific extracellular vesicle proteome defines disease-related molecules associated with activated astrocytes in Alzheimer's disease brain.

You Y, Muraoka S, Jedrychowski MP, Hu J, McQuade AK, Young-Pearse T, Aslebagh R, Shaffer SA, Gygi SP, Blurton-Jones M, Poon WW, Ikezu T
Journal of extracellular vesicles 2022 Jan;11(1):e12183

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Breast Cancer MDA-MB-231 Cells Use Secreted Heat Shock Protein-90alpha (Hsp90α) to Survive a Hostile Hypoxic Environment.

Dong H, Zou M, Bhatia A, Jayaprakash P, Hofman F, Ying Q, Chen M, Woodley DT, Li W
Scientific reports 2016 Feb 5;6:20605

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Hsp90α and Hsp90β together operate a hypoxia and nutrient paucity stress-response mechanism during wound healing.

Jayaprakash P, Dong H, Zou M, Bhatia A, O'Brien K, Chen M, Woodley DT, Li W
Journal of cell science 2015 Apr 15;128(8):1475-80

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Extracellular heat shock protein 90 signals through subdomain II and the NPVY motif of LRP-1 receptor to Akt1 and Akt2: a circuit essential for promoting skin cell migration in vitro and wound healing in vivo.

Tsen F, Bhatia A, O'Brien K, Cheng CF, Chen M, Hay N, Stiles B, Woodley DT, Li W
Molecular and cellular biology 2013 Dec;33(24):4947-59

Immunocytochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunofluorescence analysis of LRP1 was performed using 70% confluent log phase U-87 MG and SK-BR-3 cells. The cells were fixed with 4% Paraformaldehyde for 10 minutes, permeabilized with 0.1% Triton™ X-100 for 10 minutes, and blocked with 2% BSA for 2 hours at room temperature. The cells were labeled with LRP1 Monoclonal Antibody (A2MR-beta-1) (Product # 37-7600) at 0.5 µg/mL in 0.1% BSA, incubated at 4 degree celsius overnight and then labeled with Donkey anti-Mouse IgG (H+L) Highly Cross-Adsorbed Secondary Antibody, Alexa Fluor Plus 488 (Product #A32766), (1:2000 dilution) for 45 minutes at room temperature (Panel a: Green). Nuclei (Panel b: Blue) were stained with SlowFade® Gold Antifade Mountant with DAPI (Product # S36938). F-actin (Panel c: Red) was stained with Rhodamine Phalloidin (Product # R415, 1:300). Panel d represents the merged image showing nuclear localization. Panel (e-h) represents SK-BR-3 cells with respective stainings and does not show expression for LRP1. The images were captured at 60X magnification.

Other assay

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

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Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

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Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

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Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

NULL

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

NULL

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

NULL

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Figure 1 Selection of MDA-MB-231 breast cancer cell line as the model of study. Western blots for Hsp90alpha and Hsp90beta in total lysates (TL) ( A ) or conditioned media (100x) (CM) ( B ) and the invasiveness ( C ) of the indicated breast cancer and control cell lines. Western blots for LRP-1 receptor among cell lines ( D ) and the total ( E ) and the secreted ( F ) Hsp90alpha and Hsp90beta under hypoxia in MDA-MB-231. Intracellular ( G ) and secreted ( H ) ratios between Hsp90alpha and Hsp90beta in MDA-MB-231 cells.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Figure 3 Rescue of Hsp90alpha-knockout cells from hypoxia-driven killing by extracellular Hsp90alpha, but not Hsp90beta, protein via LRP-1 receptor signaling. ( A ) Extracellular Hsp90alpha, not Hsp90beta, rescues viability of Hsp90alpha-knockout cells. ( B ) Quantitation of viability data. ( C ) Evidence of purified recombinant proteins for rescues. ( D ) Down-regulation of LRP-1 shown by Western blot. ( E ) No rescue of LRP-1-downregulated cells from hypoxia by extracellular Hsp90alpha. ( F ) Quantitation . n = 3, *p < 0.05.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

FIGURE 7 The M7 module is enriched in activated astrocyte derived EV markers and involved in inflammatory processes. (a) The enrichment of proteins, identified as EV markers for the activated astrocytes, was calculated for each module in the AD protein network using a one-tailed Fisher's exact test. Both the raw p -value and the FDR p -value (correction for multiple comparisons by the Benjamini-Hochberg method) are shown; The dashed line represents a p -value