Antibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Western blot [2]
- Immunohistochemistry [1]
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Validation data
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- Product number
- MAB68871 - Provider product page
- Provider
- R&D Systems
- Product name
- Human Troponin I Antibody
- Antibody type
- Monoclonal
- Description
- Protein A or G purified from hybridoma culture supernatant. Detects human Cardiac Troponin I in direct ELISAs and Western blots.
- Reactivity
- Human
- Host
- Mouse
- Conjugate
- Unconjugated
- Isotype
- IgG
- Antibody clone number
- 679021
- Vial size
- 100 ug
- Concentration
- LYOPH
- Storage
- Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 6 months, -20 to -70 °C under sterile conditions after reconstitution.
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Supportive validation
- Submitted by
- R&D Systems (provider)
- Main image
- Experimental details
- Detection of Human Troponin I by Western Blot. Western blot shows lysates of human heart tissue. PVDF membrane was probed with 1 µg/mL of Mouse Anti-Human Troponin I Monoclonal Antibody (Catalog # MAB68871) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF007). A specific band was detected for Troponin I at approximately 24-29 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
- Submitted by
- R&D Systems (provider)
- Main image
- Experimental details
- Detection of Human Troponin I by Simple WesternTM. Simple Western lane view shows lysates of human heart tissue and human heart (ventricular) tissue, loaded at 0.5 mg/mL. Specific bands were detected for Troponin I at approximately 27-31 kDa (as indicated) using 10 µg/mL of Mouse Anti-Human Troponin I Monoclonal Antibody (Catalog # MAB68871). This experiment was conducted under reducing conditions and using the 12-230 kDa separation system.
Supportive validation
- Submitted by
- R&D Systems (provider)
- Main image
- Experimental details
- Troponin I in Human Heart. Troponin I was detected in immersion fixed paraffin-embedded sections of human heart using Mouse Anti-Human Troponin I Monoclonal Antibody (Catalog # MAB68871) at 15 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Mouse HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS002) and counterstained with hematoxylin (blue). Lower panel shows a lack of labeling when primary antibodies are omitted and tissue is stained only with secondary antibody followed by incubation with detection reagents. Specific staining was localized to cardiac muscle. View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.