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Western blot
ImmunocytochemistryAntibody data
- Antibody Data
- Antigen structure
- References [1]
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- Validations
- Western blot [1]
- Immunoprecipitation [1]
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- Product number
- NBP2-29526 - Provider product page
- Provider
- Novus Biologicals
- Product name
- Rabbit Polyclonal Troponin I Type 3 (cardiac) Antibody
- Antibody type
- Polyclonal
- Description
- Immunogen affinity purified. Specific for the ~25k cardiac troponin I protein phosphorylated at Ser150. Immunolabeling is greatly decreased with lambda-phosphatase treatment.
- Reactivity
- Mouse, Rat
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 0.1 ml
- Storage
- Store at -20C. Avoid freeze-thaw cycles.
Submitted references Pim-1 Kinase Phosphorylates Cardiac Troponin I and Regulates Cardiac Myofilament Function.
Zhu N, Yi B, Guo Z, Zhang G, Huang S, Qin Y, Zhao X, Sun J
Cellular physiology and biochemistry : international journal of experimental cellular physiology, biochemistry, and pharmacology 2018;45(6):2174-2186
Cellular physiology and biochemistry : international journal of experimental cellular physiology, biochemistry, and pharmacology 2018;45(6):2174-2186
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Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: Troponin I Type 3 (cardiac) [p Ser150] Antibody [NBP2-29526] - Western blot of mouse heart lysate showing specific immunolabeling of ~25k cTnI protein phosphorylated at Ser150 (control). Phosphospecificity is shown in the second lane. The blot is identical to the control except that the lysate was incubated in lambda-Ptase (1400 units for 30 min). The immunolabeling is greatly decreased by treatment with lambda-Ptase.
Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunoprecipitation: Troponin I Type 3 (cardiac) [p Ser150] Antibody [NBP2-29526] - Adult mouse heart tissue samples were extracted and immunoprecipitated with either anti-Pim-1 antibody or control IgG and then analyzed by Western blot. From Zhu et al.: Phosphorylation of cTnI by Pim1 Kinase, Cell Physiol Biochem 2018;45:2174-2186.
