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LearnH00007137-M04
antibody from Novus Biologicals
Targeting: TNNI3
CMD2A, CMH7, TNNC1
Western blot ELISA Immunocytochemistry
Immunoprecipitation Immunohistochemistry Blocking/NeutralizingAntibody data
- Antibody Data
- Antigen structure
- References [1]
- Comments [0]
- Validations
- Western blot [2]
- ELISA [1]
- Immunocytochemistry [1]
- Immunohistochemistry [1]
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- Product number
- H00007137-M04 - Provider product page
- Provider
- Novus Biologicals
- Proper citation
- Novus Cat#H00007137-M04, RRID:AB_548741
- Product name
- Mouse Monoclonal Troponin I Type 3 (cardiac) Antibody
- Antibody type
- Monoclonal
- Description
- IgG purified. TNNI3 - troponin I type 3 (cardiac)
- Reactivity
- Human, Rat
- Host
- Mouse
- Isotype
- IgG
- Vial size
- 0.1 mg
- Storage
- Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
Submitted references Myomegalin is a novel A-kinase anchoring protein involved in the phosphorylation of cardiac myosin binding protein C.
Uys GM, Ramburan A, Loos B, Kinnear CJ, Korkie LJ, Mouton J, Riedemann J, Moolman-Smook JC
BMC cell biology 2011 May 10;12:18
BMC cell biology 2011 May 10;12:18
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Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: Troponin I Type 3 (cardiac) Antibody (1E7) [H00007137-M04] - Western blot analysis of TNNI3 over-expressed 293 cell line, cotransfected with TNNI3 Validated Chimera RNAi or non-transfected control. Blot probed with H00007137-M04. GAPDH (36.1 kDa) used as loading control.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: Troponin I Type 3 (cardiac) Antibody (1E7) [H00007137-M04] - Analysis of TNNI3 expression in transfected 293T cell line by TNNI3 monoclonal antibody (M04), clone 1E7. Lane 1: TNNI3 transfected lysatE (24 KDa). Lane 2: Non-transfected lysate.
Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- ELISA: Troponin I Type 3 (cardiac) Antibody (1E7.) [H00007137-M04] - Troponin I Type 3 (cardiac) Antibody (1E7) [H00007137-M04] - Detection limit for recombinant GST tagged TNNI3 is 1 ng/ml as a capture antibody.
Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunocytochemistry/Immunofluorescence: Troponin I Type 3 (cardiac) Antibody (1E7) [H00007137-M04] - Analysis of monoclonal antibody to TNNI3 on HeLa cell. Antibody concentration 10 ug/ml
Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunohistochemistry-Paraffin: Troponin I Type 3 (cardiac) Antibody (1E7) [H00007137-M04] - Analysis of monoclonal antibody to TNNI3 on formalin-fixed paraffin-embedded human heart. Antibody concentration 0.7 ug/ml
