TA503404

antibody from OriGene

Targeting: ANAPC2 APC2, KIAA1406

Western blot (Supportive data in Antibodypedia)

Immunocytochemistry (Supportive data in Antibodypedia)

Antibody Data

Product number

TA503404

Provider

OriGene

Proper citation

OriGene Cat#TA503404, RRID:AB_11123806

Product name

ANAPC2 (Apc2) mouse monoclonal antibody, clone OTI1B12 (formerly 1B12)

Antibody type

Monoclonal

Description

ANAPC2 (Apc2) mouse monoclonal antibody, clone OTI1B12 (formerly 1B12)

Host

Mouse

Conjugate

Unconjugated

Epitope

ANAPC2

Isotype

IgG

Antibody clone number

OTI1B12

Vial size

100 µl

Concentration

0.82 mg/ml

Western blot

Supportive validation

Submitted by

OriGene (provider)

Main image

Experimental details

Western blot analysis of extracts (35ug) from 9 different cell lines by using anti-ANAPC2 monoclonal antibody (HepG2: human; HeLa: human; SVT2: mouse; A549: human; COS7: monkey; Jurkat: human; MDCK: canine; PC12: rat; MCF7: human).

Validation comment

WB

Supportive validation

Submitted by

OriGene (provider)

Main image

Experimental details

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY ANAPC2 (RC207539, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-ANAPC2.

Validation comment

WB

Immunocytochemistry

Supportive validation

Submitted by

OriGene (provider)

Main image

Experimental details

Anti-ANAPC2 mouse monoclonal antibody (TA503404) immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY ANAPC2(RC207539).

Validation comment

IF