Antibody data
- Antibody Data
- Antigen structure
- References [1]
- Comments [0]
- Validations
- Western blot [1]
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Validation data
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- Product number
- 600-401-432 - Provider product page
- Provider
- Rockland Immunochemicals, Inc.
- Proper citation
- Rockland Cat#600-401-432, RRID:AB_2201907
- Product name
- Anti-TGF beta 1 (RABBIT) Antibody - 600-401-432
- Antibody type
- Polyclonal
- Vial size
- 100 µl
Submitted references Sepsis-induced lung inflammation is modulated by insulin.
Filgueiras LR, Capelozzi VL, Martins JO, Jancar S
BMC pulmonary medicine 2014 Nov 15;14:177
BMC pulmonary medicine 2014 Nov 15;14:177
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Supportive validation
- Submitted by
- Rockland Immunochemicals, Inc. (provider)
- Main image
- Experimental details
- Western blot analysis using Rockland's Affinity Purified anti-TGF beta 1 antibody to detect human TGF beta 1. Each lane contains 250 ng of protein under non-reducing (lane 1) and reducing conditions (lane 2). Comparison to molecular weight markers (not shown) was used to estimate the indicated molecular weights. The blot was incubated with a 1:200 dilution of the antibody at room temperature for 1 h followed by detection using IRDye® 800 labeled Goat-a-Rabbit IgG (H&L) (611-132-122) diluted 1:2,500. IRDye® 800 fluorescence image was captured using the Odyssey® Infrared Imaging System developed by LI-COR. IRDye is a trademark of LI-COR, Inc. Other detection systems will yield similar results.
- Validation comment
- Western Blot