Antibody data
- Antibody Data
- Antigen structure
- References [56]
- Comments [0]
- Validations
- Western blot [1]
- Immunohistochemistry [2]
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Validation data
Reference
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- Product number
- 12216-1-AP - Provider product page
- Provider
- Proteintech Group
- Proper citation
- Proteintech Cat#12216-1-AP, RRID:AB_2086929
- Product name
- Cathepsin B antibody
- Antibody type
- Polyclonal
- Description
- Cathepsin B antibody (Cat. #12216-1-AP) is a rabbit polyclonal antibody that shows reactivity with human, mouse and has been validated for the following applications: FC, IF, IHC, WB, ELISA.
- Reactivity
- Human, Mouse
- Host
- Rabbit
- Conjugate
- Unconjugated
- Isotype
- IgG
- Vial size
- 20ul, 150ul
Submitted references Staphylococcus aureus promotes its intracellular survival by inhibiting Rab11-Rab11FIP4-mediated vesicle trafficking.
scRank infers drug-responsive cell types from untreated scRNA-seq data using a target-perturbed gene regulatory network.
Acetylation of MOB1 mediates polyphyllin II-reduced lysosome biogenesis in breast cancer by promoting the cytoplasmic retention of the YAP/TFEB coactivator complex.
Attenuation of pro-tumorigenic senescent secretory phenotype by StN, a novel derivative of stevioside, potentiates its inhibitory activity on hepatocellular carcinoma.
Thioredoxin-1 decreases alpha-synuclein induced by MPTP through promoting autophagy-lysosome pathway.
Fluorofenidone attenuates renal fibrosis by inhibiting lysosomal cathepsin‑mediated NLRP3 inflammasome activation.
3-Monochloropropane-1,2-diol esters induce HepG2 cells necroptosis via CTSB/TFAM/ROS pathway.
Lysine methylation of PPP1CA by the methyltransferase SUV39H2 disrupts TFEB-dependent autophagy and promotes intervertebral disc degeneration.
Ar-turmerone inhibits the proliferation and mobility of glioma by downregulating cathepsin B.
Mycobacterium tuberculosis resides in lysosome-poor monocyte-derived lung cells during chronic infection.
Dual targets of lethal apoptosis and protective autophagy in liver cancer with periplocymarin elicit a limited therapeutic effect.
Mechanistic Insights into the Role of OPN in Mediating Brain Damage via Triggering Lysosomal Damage in Microglia/Macrophage.
TFEB coordinates autophagy and pyroptosis as hepatotoxicity responses to ZnO nanoparticles.
De novo lipogenesis fuels adipocyte autophagosome and lysosome membrane dynamics.
The relationship between pepsinogen C and gastric carcinogenesis: a transgene and population study.
Mycobacterium tuberculosis resides in lysosome-poor monocyte-derived lung cells during chronic infection.
Cathepsin B mediates the lysosomal-mitochondrial apoptosis pathway in arsenic-induced microglial cell injury.
3, 14, 19-Triacetyl Andrographolide alleviates the cognitive dysfunction of 3 × Tg-AD mice by inducing initiation and promoting degradation process of autophagy.
Evocalcet Rescues Secondary Hyperparathyroidism-driven Cortical Porosity in CKD Male Rats.
The cell type dependent sorting of CD9- and CD81 to extracellular vesicles can be exploited to convey tumor sensitive cargo to target cells.
Behind the Indolent Facade: Uncovering the Molecular Features and Malignancy Potential in Lung Minimally Invasive Adenocarcinoma by Single-Cell Transcriptomics.
Blockade of Autophagy Prevents the Progression of Hyperuricemic Nephropathy Through Inhibiting NLRP3 Inflammasome-Mediated Pyroptosis.
Polystyrene micro(nano)plastics damage the organelles of RBL-2H3 cells and promote MOAP-1 to induce apoptosis.
In addition to its endosomal escape effect, platycodin D also synergizes with ribosomal inactivation protein to induce apoptosis in hepatoma cells through AKT and MAPK signaling pathways.
Cathepsin L-containing exosomes from α-synuclein-activated microglia induce neurotoxicity through the P2X7 receptor.
Methylmercury induces lysosomal membrane permeabilization through JNK-activated Bax lysosomal translocation in neuronal cells.
Inhibition of STAT3-ferroptosis negative regulatory axis suppresses tumor growth and alleviates chemoresistance in gastric cancer.
Growth differentiation factor 11 accelerates liver senescence through the inhibition of autophagy.
Lysosomal dysfunction is associated with NLRP3 inflammasome activation in chronic unpredictable mild stress-induced depressive mice.
Sodium-Glucose Co-transporter-2 Inhibitor of Dapagliflozin Attenuates Myocardial Ischemia/Reperfusion Injury by Limiting NLRP3 Inflammasome Activation and Modulating Autophagy.
CTSB Knockdown Inhibits Proliferation and Tumorigenesis in HL-60 Cells.
Autophagy Contributes to the Rapamycin-Induced Improvement of Otitis Media.
Cordycepin inhibits cell senescence by ameliorating lysosomal dysfunction and inducing autophagy through the AMPK and mTOR-p70S6K pathway.
1,3-dichloro-2-propanol induced hepatic lipid accumulation by inhibiting autophagy via AKT/mTOR/FOXO1 pathway in mice.
Exopolysaccharides from Bifidobacterium animalis Ameliorate Escherichia coli-Induced IPEC-J2 Cell Damage via Inhibiting Apoptosis and Restoring Autophagy.
MEHP induces pyroptosis and autophagy alternation by cathepsin B activation in INS-1 cells.
Curcumin analogue AI-44 alleviates MSU-induced gouty arthritis in mice via inhibiting cathepsin B-mediated NLRP3 inflammasome activation.
A proteomic approach towards understanding the pathogenesis of Mooren's ulcer.
TRIM39 deficiency inhibits tumor progression and autophagic flux in colorectal cancer via suppressing the activity of Rab7.
Cilostazol alleviate nicotine induced cardiomyocytes hypertrophy through modulation of autophagy by CTSB/ROS/p38MAPK/JNK feedback loop.
Nicotine induces cardiac toxicity through blocking mitophagic clearance in young adult rat.
Heat shock factor 4 regulates lysosome activity by modulating the αB-crystallin-ATP6V1A-mTOR complex in ocular lens.
Neurotoxic microglia promote TDP-43 proteinopathy in progranulin deficiency.
GILT restricts the cellular entry mediated by the envelope glycoproteins of SARS-CoV, Ebola virus and Lassa fever virus.
Inhibition of PI3K/mTOR increased the sensitivity of hepatocellular carcinoma cells to cisplatin via interference with mitochondrial-lysosomal crosstalk.
Alantolactone induces apoptosis and improves chemosensitivity of pancreatic cancer cells by impairment of autophagy-lysosome pathway via targeting TFEB.
Promotion of autophagosome-lysosome fusion via salvianolic acid A-mediated SIRT1 up-regulation ameliorates alcoholic liver disease.
Dietary eicosapentaenoic acid normalizes hippocampal omega-3 and 6 polyunsaturated fatty acid profile, attenuates glial activation and regulates BDNF function in a rodent model of neuroinflammation induced by central interleukin-1β administration.
NDRG1 inhibition sensitizes osteosarcoma cells to combretastatin A-4 through targeting autophagy.
A novel acridine derivative, LS-1-10 inhibits autophagic degradation and triggers apoptosis in colon cancer cells.
Adipocyte-derived exosomes promote lung cancer metastasis by increasing MMP9 activity via transferring MMP3 to lung cancer cells.
The role of NLRP3-CASP1 in inflammasome-mediated neuroinflammation and autophagy dysfunction in manganese-induced, hippocampal-dependent impairment of learning and memory ability.
Green Tea Polyphenols, Mimicking the Effects of Dietary Restriction, Ameliorate High-Fat Diet-Induced Kidney Injury via Regulating Autophagy Flux.
Expression profile of cathepsins indicates the potential of cathepsins B and D as prognostic factors in breast cancer patients.
Expression of cystatin C and its effect on EC9706 cells in esophageal carcinoma.
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3-Monochloropropane-1,2-diol esters induce HepG2 cells necroptosis via CTSB/TFAM/ROS pathway.
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Lysine methylation of PPP1CA by the methyltransferase SUV39H2 disrupts TFEB-dependent autophagy and promotes intervertebral disc degeneration.
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De novo lipogenesis fuels adipocyte autophagosome and lysosome membrane dynamics.
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The relationship between pepsinogen C and gastric carcinogenesis: a transgene and population study.
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Mycobacterium tuberculosis resides in lysosome-poor monocyte-derived lung cells during chronic infection.
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Cathepsin B mediates the lysosomal-mitochondrial apoptosis pathway in arsenic-induced microglial cell injury.
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3, 14, 19-Triacetyl Andrographolide alleviates the cognitive dysfunction of 3 × Tg-AD mice by inducing initiation and promoting degradation process of autophagy.
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Polystyrene micro(nano)plastics damage the organelles of RBL-2H3 cells and promote MOAP-1 to induce apoptosis.
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Cathepsin L-containing exosomes from α-synuclein-activated microglia induce neurotoxicity through the P2X7 receptor.
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Methylmercury induces lysosomal membrane permeabilization through JNK-activated Bax lysosomal translocation in neuronal cells.
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Inhibition of STAT3-ferroptosis negative regulatory axis suppresses tumor growth and alleviates chemoresistance in gastric cancer.
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Growth differentiation factor 11 accelerates liver senescence through the inhibition of autophagy.
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Lysosomal dysfunction is associated with NLRP3 inflammasome activation in chronic unpredictable mild stress-induced depressive mice.
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FEBS open bio 2021 Oct;11(10):2705-2714
1,3-dichloro-2-propanol induced hepatic lipid accumulation by inhibiting autophagy via AKT/mTOR/FOXO1 pathway in mice.
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Food and chemical toxicology : an international journal published for the British Industrial Biological Research Association 2021 Nov;157:112578
Exopolysaccharides from Bifidobacterium animalis Ameliorate Escherichia coli-Induced IPEC-J2 Cell Damage via Inhibiting Apoptosis and Restoring Autophagy.
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MEHP induces pyroptosis and autophagy alternation by cathepsin B activation in INS-1 cells.
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Experimental eye research 2021 Apr;205:108509
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Cell death & disease 2021 Apr 12;12(4):391
Cilostazol alleviate nicotine induced cardiomyocytes hypertrophy through modulation of autophagy by CTSB/ROS/p38MAPK/JNK feedback loop.
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International journal of biological sciences 2020;16(11):2001-2013
Nicotine induces cardiac toxicity through blocking mitophagic clearance in young adult rat.
Jia G, Meng Z, Liu C, Ma X, Gao J, Liu J, Guo R, Yan Z, Christopher T, Lopez B, Liu W, Dai H, Lau WB, Jiao X, Zhao J, Wang ZX, Cao J, Wang Y
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Life sciences 2020 Sep 15;257:118084
Heat shock factor 4 regulates lysosome activity by modulating the αB-crystallin-ATP6V1A-mTOR complex in ocular lens.
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Biochimica et biophysica acta. General subjects 2020 Mar;1864(3):129496
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Zhang J, Velmeshev D, Hashimoto K, Huang YH, Hofmann JW, Shi X, Chen J, Leidal AM, Dishart JG, Cahill MK, Kelley KW, Liddelow SA, Seeley WW, Miller BL, Walther TC, Farese RV Jr, Taylor JP, Ullian EM, Huang B, Debnath J, Wittmann T, Kriegstein AR, Huang EJ
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Nature 2020 Dec;588(7838):459-465
GILT restricts the cellular entry mediated by the envelope glycoproteins of SARS-CoV, Ebola virus and Lassa fever virus.
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Emerging microbes & infections 2019;8(1):1511-1523
Inhibition of PI3K/mTOR increased the sensitivity of hepatocellular carcinoma cells to cisplatin via interference with mitochondrial-lysosomal crosstalk.
Sheng J, Shen L, Sun L, Zhang X, Cui R, Wang L
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Cell proliferation 2019 May;52(3):e12609
Alantolactone induces apoptosis and improves chemosensitivity of pancreatic cancer cells by impairment of autophagy-lysosome pathway via targeting TFEB.
He R, Shi X, Zhou M, Zhao Y, Pan S, Zhao C, Guo X, Wang M, Li X, Qin R
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Toxicology and applied pharmacology 2018 Oct 1;356:159-171
Promotion of autophagosome-lysosome fusion via salvianolic acid A-mediated SIRT1 up-regulation ameliorates alcoholic liver disease.
Shi X, Sun R, Zhao Y, Fu R, Wang R, Zhao H, Wang Z, Tang F, Zhang N, Tian X, Yao J
RSC advances 2018 May 30;8(36):20411-20422
RSC advances 2018 May 30;8(36):20411-20422
Dietary eicosapentaenoic acid normalizes hippocampal omega-3 and 6 polyunsaturated fatty acid profile, attenuates glial activation and regulates BDNF function in a rodent model of neuroinflammation induced by central interleukin-1β administration.
Dong Y, Xu M, Kalueff AV, Song C
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European journal of nutrition 2018 Aug;57(5):1781-1791
NDRG1 inhibition sensitizes osteosarcoma cells to combretastatin A-4 through targeting autophagy.
Wang H, Li W, Xu J, Zhang T, Zuo D, Zhou Z, Lin B, Wang G, Wang Z, Sun W, Sun M, Chang S, Cai Z, Hua Y
Cell death & disease 2017 Sep 14;8(9):e3048
Cell death & disease 2017 Sep 14;8(9):e3048
A novel acridine derivative, LS-1-10 inhibits autophagic degradation and triggers apoptosis in colon cancer cells.
Fu W, Li X, Lu X, Zhang L, Li R, Zhang N, Liu S, Yang X, Wang Y, Zhao Y, Meng X, Zhu WG
Cell death & disease 2017 Oct 5;8(10):e3086
Cell death & disease 2017 Oct 5;8(10):e3086
Adipocyte-derived exosomes promote lung cancer metastasis by increasing MMP9 activity via transferring MMP3 to lung cancer cells.
Wang J, Wu Y, Guo J, Fei X, Yu L, Ma S
Oncotarget 2017 Oct 10;8(47):81880-81891
Oncotarget 2017 Oct 10;8(47):81880-81891
The role of NLRP3-CASP1 in inflammasome-mediated neuroinflammation and autophagy dysfunction in manganese-induced, hippocampal-dependent impairment of learning and memory ability.
Wang D, Zhang J, Jiang W, Cao Z, Zhao F, Cai T, Aschner M, Luo W
Autophagy 2017 May 4;13(5):914-927
Autophagy 2017 May 4;13(5):914-927
Green Tea Polyphenols, Mimicking the Effects of Dietary Restriction, Ameliorate High-Fat Diet-Induced Kidney Injury via Regulating Autophagy Flux.
Xie X, Yi W, Zhang P, Wu N, Yan Q, Yang H, Tian C, Xiang S, Du M, Getachew Assefa E, Zuo X, Ying C
Nutrients 2017 May 14;9(5)
Nutrients 2017 May 14;9(5)
Expression profile of cathepsins indicates the potential of cathepsins B and D as prognostic factors in breast cancer patients.
Sun T, Jiang D, Zhang L, Su Q, Mao W, Jiang C
Oncology letters 2016 Jan;11(1):575-583
Oncology letters 2016 Jan;11(1):575-583
Expression of cystatin C and its effect on EC9706 cells in esophageal carcinoma.
Yan Y, Zhou K, Wang L, Zhou Y, Chen X, Fan Q
International journal of clinical and experimental pathology 2015;8(9):10102-11
International journal of clinical and experimental pathology 2015;8(9):10102-11
Host-derived, pore-forming toxin-like protein and trefoil factor complex protects the host against microbial infection.
Xiang Y, Yan C, Guo X, Zhou K, Li S, Gao Q, Wang X, Zhao F, Liu J, Lee WH, Zhang Y
Proceedings of the National Academy of Sciences of the United States of America 2014 May 6;111(18):6702-7
Proceedings of the National Academy of Sciences of the United States of America 2014 May 6;111(18):6702-7
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Supportive validation
- Submitted by
- Proteintech Group (provider)
- Main image
- Experimental details
- A375 cells were subjected to SDS PAGE followed by western blot with 12216-1-AP(CTSB antibody) at dilution of 1:800
- Sample type
- cell line
Supportive validation
- Submitted by
- Proteintech Group (provider)
- Main image
- Experimental details
- Immunohistochemical of paraffin-embedded human breast cancer using 12216-1-AP(CTSB antibody) at dilution of 1:50 (under 10x lens)
- Sample type
- tissue
- Submitted by
- Proteintech Group (provider)
- Main image
- Experimental details
- The Cathepsin B antibody from Proteintech is a rabbit polyclonal antibody to a recombinant protein of human Cathepsin B. This antibody recognizes human, mouse antigen. The Cathepsin B antibody has been validated for the following applications: ELISA, WB, IHC analysis.