PA5-17006

antibody from Invitrogen Antibodies

Targeting: CTSB

Antibody data

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Validation data

CTSB protein structure - PA5-17006 shown in red.

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Western blot analysis was performed on whole cell extracts (30 µg lysate) of T-47D (Lane 1), MDA-MB-231 (Lane 2) and THP-1 (Lane 3). The blot was probed with anti-Cathepsin B Rabbit Polyclonal Antibody (Product # PA5-17006, 1:500 dilution) and detected by chemiluminescence using Goat anti Rabbit IgG (H+L) Superclonal™ Secondary Antibody, HRP conjugate (Product # A27036, 0.25 µg/mL, 1:4000 dilution). A 37 kDa band corresponding to Cathepsin B was detected across the cell lines tested.

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Western blot analysis of Cathepsin B in extracts from RPM18226 and HT-1080 cell lines using Cathepsin B polyclonal antibody (Product # PA5-17006).

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Western blot analysis of Cathepsin B in extracts from secreted protein from HL-60 cells, untreated or treated overnight with TPA (20 nM), using Cathepsin B polyclonal antibody (Product # PA5-17006).

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Knockdown of Cathepsin B was achieved by transfecting MCF7 cells with Cathepsin B specific siRNAs (Silencer® select Product # s3739). Western blot analysis (Fig. a) was performed using whole cell extracts from Cathepsin B knockdown cells (lane 3), non-specific scrambled siRNA transfected cells (lane 2) and untransfected cells (lane 1). The blots were probed with Cathepsin B Polyclonal Antibody (Product # PA5-17006, 1:500 dilution) and Goat anti-Rabbit IgG (H+L) Superclonal™ Secondary Antibody, HRP conjugate (Product # A27036, 0.25 µg/mL, 1:4000 dilution). Densitometric analysis of this western blot is shown in histogram (Fig. b). Loss of signal upon siRNA mediated knock down confirms that antibody is specific to Cathepsin B.