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Western blot
ELISAAntibody data
- Antibody Data
- Antigen structure
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- Validations
- Western blot [2]
- Immunohistochemistry [5]
- Flow cytometry [1]
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- Product number
- RQ6856 - Provider product page
- Provider
- NSJ Bioreagents
- Product name
- CTSB Antibody / Cathepsin B
- Antibody type
- Polyclonal
- Description
- This highly specific CTSB antibody is suitable for use in Western blot/Flow cytometry/Immunohistochemistry/Direct ELISA applications with human, mouse and rat samples.
- Reactivity
- Human, Mouse, Rat
- Host
- Rabbit
- Conjugate
- Unconjugated
- Vial size
- 100 ug
- Concentration
- 0.5mg/ml if reconstituted with 0.2ml sterile DI water
- Storage
- After reconstitution, the CTSB antibody can be stored for up to one month at 4oC. For long-term, aliquot and store at -20oC. Avoid repeated freezing and thawing.
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Supportive validation
- Submitted by
- NSJ Bioreagents (provider)
- Main image
- Experimental details
- Western blot testing of human 1) U-87 MG and 2) HepG2 cell lysate with CTSB antibody. Molecular weight: 38-46 kDa depending on glycosylation level. An ~31 kDa form (propeptide removed) may be observed and may be further processed into an ~25 kDa heavy chain and ~5 kDa light chain.
- Submitted by
- NSJ Bioreagents (provider)
- Main image
- Experimental details
- Western blot testing of 1) human U-87 MG, 2) human HepG2, 3) human A549, 4) rat brain, 5) rat liver and 6) mouse liver tissue lysate with CTSB antibody. Molecular weight: 38-46 kDa depending on glycosylation level. An ~31 kDa form (propeptide removed) may be observed and may be further processed into an ~25 kDa heavy chain and ~5 kDa light chain.
Supportive validation
- Submitted by
- NSJ Bioreagents (provider)
- Main image
- Experimental details
- IHC staining of FFPE human liver cancer tissue with CTSB antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.
- Submitted by
- NSJ Bioreagents (provider)
- Main image
- Experimental details
- IHC staining of FFPE human rectal cancer tissue with CTSB antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.
- Submitted by
- NSJ Bioreagents (provider)
- Main image
- Experimental details
- IHC staining of FFPE human thyroid cancer tissue with CTSB antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.
- Submitted by
- NSJ Bioreagents (provider)
- Main image
- Experimental details
- IHC staining of FFPE mouse liver cancer tissue with CTSB antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.
- Submitted by
- NSJ Bioreagents (provider)
- Main image
- Experimental details
- IHC staining of FFPE rat liver cancer tissue with CTSB antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.
Supportive validation
- Submitted by
- NSJ Bioreagents (provider)
- Main image
- Experimental details
- Flow cytometry testing of human SiHa cells with CTSB antibody at 1ug/million cells (blocked with goat sera); Red=cells alone, Green=isotype control, Blue= CTSB antibody.
