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Western blot
ImmunohistochemistryAntibody data
- Antibody Data
- Antigen structure
- References [1]
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- Validations
- Immunohistochemistry [1]
- Flow cytometry [2]
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- Product number
- PA5-14255 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- Cathepsin B Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Synthetic peptide
- Description
- This antibody is predicted to react with non-human primate based on sequence homology.
- Reactivity
- Human
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 400 μL
- Concentration
- 0.5 mg/mL
- Storage
- Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
Submitted references L-leucyl-L-leucine methyl ester does not release cysteine cathepsins to the cytosol but inactivates them in transiently permeabilized lysosomes.
Repnik U, Borg Distefano M, Speth MT, Ng MYW, Progida C, Hoflack B, Gruenberg J, Griffiths G
Journal of cell science 2017 Sep 15;130(18):3124-3140
Journal of cell science 2017 Sep 15;130(18):3124-3140
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemistry analysis of Cathepsin B in formalin-fixed and paraffin-embedded human hepatocarcinoma. Samples were incubated with Cathepsin B polyclonal antibody (Product # PA5-14255) which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Flow cytometry analysis of HepG2 cells using a CATB polyclonal antibody (Product # PA5-14255) (bottom), compared to a negative control cell (top) at a dilution of 1:10-50, followed by a FITC-conjugated goat anti-rabbit antibody
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Flow cytometry of Cathepsin B in HepG2 cells (bottom histogram). Samples were incubated with Cathepsin B polyclonal antibody (Product # PA5-14255) followed by FITC-conjugated goat-anti-rabbit secondary antibody. Negative control cell (top histogram).
