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- Product number
- 415500 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- Cathepsin L Monoclonal Antibody (3G10)
- Antibody type
- Monoclonal
- Antigen
- Purifed from natural sources
- Reactivity
- Human, Mouse
- Host
- Mouse
- Isotype
- IgG
- Antibody clone number
- 3G10
- Vial size
- 200 µL
- Concentration
- 0.5 mg/mL
- Storage
- -20°C
Submitted references Proteome-wide Map of Targets of T790M-EGFR-Directed Covalent Inhibitors.
Niessen S, Dix MM, Barbas S, Potter ZE, Lu S, Brodsky O, Planken S, Behenna D, Almaden C, Gajiwala KS, Ryan K, Ferre R, Lazear MR, Hayward MM, Kath JC, Cravatt BF
Cell chemical biology 2017 Nov 16;24(11):1388-1400.e7
Cell chemical biology 2017 Nov 16;24(11):1388-1400.e7
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis was performed on whole cell extracts (30 µg lysate) of MCF7 (Lane 1) and T-47D (Lane 2). The blot was probed with Anti- Cathepsin L Mouse Monoclonal Antibody (Product # 415500, 1µg/mL) and detected by chemiluminescence using Goat anti-Mouse IgG (H+L) Superclonal™ Secondary Antibody, HRP conjugate (Product # A28177, 0.25 µg/mL, 1:4000 dilution). A 42 kDa band corresponding to Cathepsin L was detected across the cell lines tested.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Knockdown of Cathepsin L was achieved by transfecting A549 with Cathepsin L specific siRNAs (Silencer® select Product # s3754). Western blot analysis (Fig. a) was performed using whole cell extracts from the A549 knockdown cells (lane 3), non-specific scrambled siRNA transfected cells (lane 2) and untransfected cells (lane 1). The blots were probed with Cathepsin L Monoclonal Antibody (3G10) (Product # 415500, 1µg/mL) and Goat anti-Mouse IgG (H+L) Superclonal™ Secondary Antibody, HRP conjugate (Product # A28177, 0.25µg/mL, 1:4000 dilution). Densitometric analysis of this western blot is shown in histogram (Fig. b). Loss of signal upon siRNA mediated knock down confirms that antibody is specific to Cathepsin L.
