MA5-23891

antibody from Invitrogen Antibodies

Targeting: CTSL CTSL1, FLJ31037

Western blot

Immunoprecipitation

Immunohistochemistry

Antibody data

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Validation data

Product number: MA5-23891 - Provider product page
Provider: Invitrogen Antibodies
Product name: Cathepsin L Monoclonal Antibody (204101)
Antibody type: Monoclonal
Antigen: Recombinant full-length protein
Description: In direct ELISAs and Western blots, no cross-reactivity with recombinant human Cathepsin B, C, L2, O, S, or X/Z/P is observed. In Western blots, both the pro and active forms of recombinant human and mouse Cathspsin L are recognized. Reconstitute at 0.5 mg/mL in sterile PBS.
Reactivity: Human, Mouse
Host: Rat
Isotype: IgG
Antibody clone number: 204101
Vial size: 100 µg
Concentration: 0.5 mg/mL
Storage: -20° C, Avoid Freeze/Thaw Cycles

CTSL protein structure - MA5-23891 shown in red.

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Figure 3 Effect of DHT and diet on cardiac SARS-CoV-2 viral entry proteins expression. Animals were treated with dihydrotestosterone (DHT) or vehicle (Veh) and maintained in low (LFD) or high (HFD) fat diet for 90 days. Ace2 ( A ), Tmprss2 ( B ), furin ( C ), cathepsin L ( D ), and ADAM17 ( E ) mRNA was quantified by RT-qPCR and standardized to the geometric mean of four housekeeping genes (HGMK) ( N = 6/group). ACE2 ( F ), TMPRSS2 ( G ), furin ( H ), cathepsin L ( I ), and ADAM17 ( J ) protein was quantified by Western-blot and normalized to GAPDH ( N = 4/group). Total furin ( H ) was quantified as the sum of profurin (dashed arrow) and cleaved soluble furin (solid arrow). Data are expressed as mean +- SEM. Data were analyzed by two-way ANOVA followed by Fisher's LSD test. a p < 0.05 vs. LFD-Veh; b p < 0.05 vs. HFD-Veh; c p < 0.05 vs. LFD-DHT.

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Figure 4 Effect of DHT and diet on renal SARS-CoV-2 viral entry proteins expression. Animals were treated with dihydrotestosterone (DHT) or vehicle (Veh) and maintained in low (LFD) or high (HFD) fat diet for 90 days. Ace2 ( A ), Tmprss2 ( B ), Tmprss4 ( C ), furin ( D ), cathepsin L ( E ), and ADAM17 ( F ) mRNA was quantified by RT-qPCR and standardized to the geometric mean of four housekeeping genes (HGMK) ( N = 6-8/group). ACE2 ( G ), TMPRSS2 ( H ), TMPRSS4 ( I ), furin ( J ), cathepsin L ( K ), and ADAM17 ( L ) protein was quantified by Western-blot and normalized to GAPDH ( N = 4/group). Total furin ( J ) was quantified as the sum of profurin (dashed arrow) and cleaved soluble furin (solid arrow). Data are expressed as mean +- SEM. Data were analyzed by two-way ANOVA followed by Fisher's LSD test. a p < 0.05 vs. LFD-Veh; b p < 0.05 vs. LFD-Veh; c p < 0.05 vs. LFD-DHT.