PA5-47971

antibody from Invitrogen Antibodies

Targeting: CTSL CTSL1, FLJ31037

Western blot (Supportive data in Antibodypedia)

Immunohistochemistry (Supportive data in Antibodypedia)

Antibody Data

Product number

PA5-47971

Provider

Invitrogen Antibodies

Product name

Cathepsin L Polyclonal Antibody

Antibody type

Polyclonal

Antigen

Recombinant full-length protein

Description

In sandwich ELISAs, less than 0.2% cross-reactivity with recombinant mouse Cathepsin L, recombinant human (rh) Cathepsin A, rhCathepsin B, rhCathepsin C, rhCathepsin D, rhCathepsin E, rhCathepsin S, and rhCathepsin V is observed.

Concentration

0.2 mg/mL

Western blot

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Western blot analysis of Cathepsin L in 10 ng recombinant Human Cathepsin L, recombinant Mouse Cathepsin L, recombinant Human Cathepsin V, recombinant Human Cathepsin K, recombinant Human Cathepsin S, recombinant Human Cathepsin H, and recombinant Human Cathepsin F. Samples were incubated in Cathepsin L polyclonal antibody (Product # PA5-47971) using a dilution of 0.1 µg/mL followed by a HRP-conjugated Anti-Goat IgG secondary antibody. A specific band was detected for Cathepsin L at approximately 35 kDa (as indicated). This experiment was conducted under reducing conditions.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Western blot analysis of Cathepsin L in 10 ng recombinant Human Cathepsin L, recombinant Mouse Cathepsin L, recombinant Human Cathepsin V, recombinant Human Cathepsin K, recombinant Human Cathepsin S, recombinant Human Cathepsin H, and recombinant Human Cathepsin F. Samples were incubated in Cathepsin L polyclonal antibody (Product # PA5-47971) using a dilution of 0.1 µg/mL followed by a HRP-conjugated Anti-Goat IgG secondary antibody. A specific band was detected for Cathepsin L at approximately 35 kDa (as indicated). This experiment was conducted under reducing conditions.

Immunohistochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemical analysis of Cathepsin L in immersion fixed paraffin-embedded sections of human kidney. Samples were incubated in Cathepsin L polyclonal antibody (Product # PA5-47971) using a dilution of 15 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Goat HRP-DAB Cell & Tissue Staining Kit (brown) and counterstained with hematoxylin (blue). Specific staining was localized to convoluted tubules.