ALX-804-293-C200

antibody from Enzo Life Sciences

Targeting: CTSL CTSL1, FLJ31037

Western blot (Data presented on provider website)

ELISA (Data presented on provider website)

Immunocytochemistry (Supportive data in Antibodypedia)

Antibody Data

Product number

ALX-804-293-C200

Provider

Enzo Life Sciences

Proper citation

Enzo Life Sciences Cat#ALX-804-293-C200, RRID:AB_2050970

Product name

Cathepsin L/procathepsin L monoclonal antibody (CPLH 3G10)

Antibody type

Monoclonal

Antigen

Purified from natural sources

Reactivity

Human, Mouse, Rat

Host

Mouse

Isotype

IgG

Antibody clone number

CPLH 3G10

Vial size

200 μg

Storage

+4°C

Handling

Do not freeze.

References

Submitted references

Immunohistochemical and clinical evaluation of cathepsin expression in soft tissue sarcomas.

Würl P, Taubert H, Meye A, Dansranjavin T, Weber E, Günther D, Berger D, Schmidt H, Dralle H, Rath FW
Virchows Archiv : an international journal of pathology 1997 Mar;430(3):221-5

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Monoclonal antibodies against cathepsin L and procathepsin L of different species.

Weber E, Bahn H, Günther D
Hybridoma 1997 Apr;16(2):159-66

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Hybridoma cells producing antibodies to cathepsin L have greatly reduced potential for tumour growth.

Weber E, Günther D, Laube F, Wiederanders B, Kirschke H
Journal of cancer research and clinical oncology 1994;120(9):564-7

Immunocytochemistry

Supportive validation

Submitted by

Enzo Life Sciences (provider)

Main image

Experimental details

Western blot (ECL) of purified mouse procathepsin L (150ng per lane). Antibody dilutions: Lane 1: 1:20'000; Lane 2: 1:50'000; Lane 3: 1:100'000; Lane 4: 1:200'000.

Supportive validation

Submitted by

Enzo Life Sciences (provider)

Main image

Experimental details

Western blot (ECL) of purified mouse cathepsin L (220ng per lane). Antibody dilution: Lane 1: 1:5'000; Lane 2: 1:10'000; Lane 3: 1:20'000; Lane 4: 1:50'000.

Supportive validation

Submitted by

Enzo Life Sciences (provider)

Main image

Experimental details

Western blot (ECL) of mouse procathepsin L in culture supernatant of spleen cells containing 10% FCS. Antibody dilutions: Lane 1: 1:20'000; Lane 2: 1:50'000; Lane 3: 1:100'000.

Supportive validation

Submitted by

Enzo Life Sciences (provider)

Main image

Experimental details

Western blot (NBT) of mouse procathepsin L in culture supernatant of spleen cells containing 10% FCS. Antibody dilutions: Lane 1: 1:50; Lane 2: 1:200; Lane 3: 1:1'000; Lane 4: 1:2'500; Lane 5: 1:10'000.

Supportive validation

Submitted by

Enzo Life Sciences (provider)

Main image

Experimental details

Figure 6: The antibody can be used as catcher antibody (5µg per ml) in an ELISA for the quantitative determination of mouse cathepsin L (standard curve between 4ng and 500ng cathepsin L per ml).