PA5-46909

antibody from Invitrogen Antibodies

Targeting: CSTB CST6, EPM1, PME, STFB

Western blot (Supportive data in Antibodypedia)

Antibody Data

Product number

PA5-46909

Provider

Invitrogen Antibodies

Product name

Cystatin B Polyclonal Antibody

Antibody type

Polyclonal

Antigen

Recombinant full-length protein

Description

In Western blots, less than 2% cross-reactivity with recombinant human (rh) Cystatin A, recombinant mouse Cystatin B, rhCystatin C, rhCystatin D, rhCystatin E/M, rhCystatin S, rhCystatin SA, and rhCystatin SN is observed.

Concentration

0.2 mg/mL

Western blot

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Western blot analysis from lysates of human colon tissue, PC-12 rat adrenal pheochromocytoma cell line, A549 human lung carcinoma cell line, and SK-BR-3 human breast cancer cell line. PVDF membrane was probed with 1 µg/mL of Goat Anti-human/Rat Cystatin B Antigen Affinity-purified Polyclonal Antibody (Product # PA5-46909) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody. A specific band was detected for Cystatin B at approximately 11 kDa (as indicated). This experiment was conducted under reducing conditions.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Knockout validation by Western blot analysis of Cystatin B in lysates of HEK293T human embryonic kidney parental cell line and Cystatin B knockout HEK293T cell line (KO). Samples were incubated in Cystatin B polyclonal antibody (Product # PA5-46909) using a dilution of 1 µg/mL followed by a HRP-conjugated Anti-Goat IgG secondary antibody. A specific band was detected for Cystatin B at approximately 12 kDa (as indicated) in the parental HEK293T cell line, but is not detectable in knockout HEK293T cell line. GAPDH is shown as a loading control. This experiment was conducted under reducing conditions.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Western blot analysis of Cystatin B in human colon tissue, PC‚12 rat adrenal pheochromocytoma cell line, A549 human lung carcinoma cell line, and SK‚BR‚3 human breast cancer cell line. Samples were incubated in Cystatin B polyclonal antibody (Product # PA5-46909) using a dilution of 1 µg/mL followed by a HRP-conjugated Anti-Goat IgG secondary antibody. A specific band was detected for Cystatin B at approximately 11 kDa (as indicated). This experiment was conducted under reducing conditions.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Western blot analysis of Cystatin B in 0.2 mg/mL lysates of A549 human lung carcinoma cell line. Samples were incubated in Cystatin B polyclonal antibody (Product # PA5-46909) using a dilution of 10 µg/mL followed by HRP-conjugated Anti-Goat IgG at a dilution of 0.0763888888888889. A specific band was detected for Cystatin B at approximately 18 kDa (as indicated)This experiment was conducted under reducing conditions and using the 12-230 kDa separation system.