LS-C756664

antibody from LSBio

Targeting: CSTB CST6, EPM1, PME, STFB

Western blot (Data presented on provider website)

Immunocytochemistry (Recommended by provider)

Immunohistochemistry (Supportive data in Antibodypedia)

Antibody Data

Product number

LS-C756664

Provider

LSBio

Product name

CSTB / Cystatin B / Stefin B Antibody LS-C756664

Antibody type

Monoclonal

Description

Immunogen affinity purified

Reactivity

Human

Host

Mouse

Isotype

IgG

Storage

After reconstitution, may be stored at 4°C for 1 month. For long-term storage and to avoid freeze-thaw cycles, aliquot and store at -20°C.

Immunohistochemistry

Supportive validation

Submitted by

LSBio (provider)

Main image

Experimental details

IHC analysis of Stefin B using anti-Stefin B antibody. Stefin B was detected in paraffin-embedded section of human tonsil tissue. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2µg/ml mouse anti-Stefin B Antibody overnight at 4°C. Biotinylated goat anti-mouse IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) with DAB as the chromogen.