Antibody data
- Antibody Data
- Antigen structure
- References [1]
- Comments [0]
- Validations
- Western blot [2]
- Immunohistochemistry [1]
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Validation data
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- Product number
- MAB7678 - Provider product page
- Provider
- Novus Biologicals
- Product name
- Mouse Monoclonal Aldo-keto Reductase 1C3/AKR1C3 Antibody
- Antibody type
- Monoclonal
- Description
- Protein A or G purified from hybridoma culture supernatant. Detects human Aldo-keto Reductase 1C3/AKR1C3 in ELISAs and Western Blot.
- Reactivity
- Human
- Host
- Mouse
- Conjugate
- Unconjugated
- Isotype
- IgG
- Vial size
- 100 ug
- Concentration
- LYOPH
- Storage
- Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 degreesC as supplied. 1 month, 2 to 8 degreesC under sterile conditions after reconstitution. 6 months, -20 to -70 degreesC under sterile conditions after reconstitution.
Submitted references Androgenic potential of human fetal adrenals at the end of the first trimester.
Savchuk I, Morvan ML, Antignac JP, Gemzell-Danielsson K, Le Bizec B, Söder O, Svechnikov K
Endocrine connections 2017 Aug;6(6):348-359
Endocrine connections 2017 Aug;6(6):348-359
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Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Detection of Human Aldo-keto Reductase 1C3/AKR1C3 by Western Blot. Western blot shows lysates of A549 human lung carcinoma cell line and HepG2 human hepatocellular carcinoma cell line. PVDF membrane was probed with 0.5 µg/mL of Mouse Anti-Human Aldo-keto Reductase 1C3/AKR1C3 Monoclonal Antibody (Catalog # MAB7678) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF018). A specific band was detected for Aldo-keto Reductase 1C3/AKR1C3 at approximately 36 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Detection of Human Aldo-keto Reductase 1C3/AKR1C3 by Simple WesternTM. Simple Western lane view shows lysates of A549 human lung carcinoma cell line and HepG2 human hepatocellular carcinoma cell line, loaded at 0.5 mg/mL. A specific band was detected for Aldo-keto Reductase 1C3/AKR1C3 at approximately 45 kDa (as indicated) using 5 µg/mL of Mouse Anti-Human Aldo-keto Reductase 1C3/AKR1C3 Monoclonal Antibody (Catalog # MAB7678) . This experiment was conducted under reducing conditions and using the 12-230 kDa separation system.
Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Aldo-keto Reductase 1C3/AKR1C3 in Human Prostate. Aldo-keto Reductase 1C3/AKR1C3 was detected in immersion fixed paraffin-embedded sections of human prostate using Mouse Anti-Human Aldo-keto Reductase 1C3/AKR1C3 Monoclonal Antibody (Catalog # MAB7678) at 1.7 µg/mL for 1 hour at room temperature followed by incubation with the Anti-Mouse IgG VisUCyte™ HRP Polymer Antibody (Catalog # VC001). Tissue was stained using DAB (brown) and counterstained with hematoxylin (blue). Specific staining was localized to cytoplasm in epithelial cells. View our protocol for IHC Staining with VisUCyte HRP Polymer Detection Reagents.