PA5-95926

antibody from Invitrogen Antibodies

Targeting: AKR1C3 DDX, HAKRB, HSD17B5, KIAA0119, PGFS

Western blot (Supportive data in Antibodypedia)

ELISA (Recommended by provider)

Immunocytochemistry (Supportive data in Antibodypedia)

Immunoprecipitation (Supportive data in Antibodypedia)

Other assay (Supportive data in Antibodypedia)

Antibody Data

Product number

PA5-95926

Provider

Invitrogen Antibodies

Product name

AKR1C3 Polyclonal Antibody

Antibody type

Polyclonal

Antigen

Recombinant full-length protein

Description

Immunogen sequence: MDSKHQCVKL NDGHFMPVLG FGTYAPPEVP RSKALEVTKL AIEAGFRHID SAHLYNNEEQ VGLAIRSKIA DGSVKREDIF YTSKLWSTFH RPELVRPALE NSLKKAQLDY VDLYLIHSPM SLKPGEELSP TDENGKVIFD IVDLCTTWEA MEKCKDAGLA KSIGVSNFNR RQLEMILNKP GLKYKPVCNQ VECHPYFNRS KLLDFCKSKD IVLVAYSALG SQRDKRWVDP NSPVLLEDPV LCALAKKHKR TPALIALRYQ LQRGVVVLAK SYNEQRIRQN VQVFEFQLTA EDMKAIDGLD RNLHYFNSDS FASHPNYPYS DEY; Positive Samples: U-87MG, 293T, LO2, A-549, DU145, Mouse kidney; Cellular Location: Cytoplasm

Reactivity

Human, Mouse

Host

Rabbit

Isotype

IgG

Vial size

100 µL

Concentration

0.6 mg/mL

Storage

-20°C, Avoid Freeze/Thaw Cycles

Western blot

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Western blot analysis of AKR1C3 in various lysates. Samples were incubated with AKR1C3 Polyclonal antibody (Product # PA5-95926) using a dilution of 1:1,000, followed by HRP Goat Anti-Rabbit IgG (H+L) at a dilution of 1:10,000. Lysates/proteins: 25 µg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 10s.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Western blot was performed using Anti-AKR1C3 Polyclonal Antibody (Product # PA5-95926) and a 37 kDa band corresponding to 17-beta-HSD 5; AKR1C3 was observed across the panel tested except for LNCaP and T-47D which are reported to be low expressors of AKR1C3. Whole cell extracts (30 µg lysate) of PC-3 (Lane 1), DU 145 (Lane 2), LNCaP (Lane 3), MCF7 (Lane 4), T-47D (Lane 5), Hep G2 (Lane 6), A549 (Lane 7), Mouse Prostate (Lane 8) were electrophoresed using NuPAGE™ 4-12% Bis-Tris Protein Gel (Product # NP0321BOX), 10 well. Resolved proteins were then transferred onto a nitrocellulose membrane (Product # IB23001) by iBlot® 2 Dry Blotting System (Product # IB21001). The blot was probed with the primary antibody (1:1000 dilution) and detected by chemiluminescence with Goat anti-Rabbit IgG (Heavy Chain) Superclonal™ Recombinant Secondary Antibody, HRP (Product # A27036, 1:20,000 dilution) using the iBright™ FL1500 Imaging System (Product # A44115). Chemiluminescent detection was performed using SuperSignal™ West Pico PLUS Chemiluminescent Substrate (Product # 34580).

Immunocytochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunofluorescence analysis of AKR1C3 in NIH/3T3 cells. Samples were incubated with AKR1C3 Polyclonal antibody (Product # PA5-95926) using a dilution of 1:100. Blue: DAPI for nuclear staining.

Immunoprecipitation

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunoprecipitation of AKR1C3 in 200 μg extracts of K-562 cells. Samples were precipitated with 3 μg AKR1C3 Polyclonal antibody (Product # PA5-95926). Western blot was performed from the immunoprecipitate using AKR1C3 Polyclonal antibody (Product # PA5-95926) at a dilution of 1:1,000.

Other assay

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunoprecipitation analysis of AKR1C3 was performed in 200 µg extracts of K-562 cells using AKR1C3 Polyclonal Antibody (Product # PA5-95926). Western blot was performed from the immunoprecipitate using AKR1C3 Polyclonal Antibody.