Explore
Validate
Learn
Western blot
ImmunoprecipitationAntibody data
- Antibody Data
- Antigen structure
- References [1]
- Comments [0]
- Validations
- Western blot [2]
- Immunocytochemistry [1]
- Other assay [1]
Submit
Validation data
Reference
Comment
Report error
- Product number
- PA5-28065 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- AKR1C3 Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Synthetic peptide
- Description
- Recommended positive controls: HepG2, HeLa, AKR1C3shRNA-transfected HeLa, rat liver. Store product as a concentrated solution. Centrifuge briefly prior to opening the vial.
- Reactivity
- Human, Rat
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 100 µL
- Concentration
- 1 mg/mL
- Storage
- Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
Submitted references The impact of flutamide on prostaglandin F(2α) synthase and prostaglandin F(2α) receptor expression, and prostaglandin F(2α) concentration in the porcine corpus luteum of pregnancy.
Grzesiak M, Knapczyk-Stwora K, Slomczynska M
Domestic animal endocrinology 2017 Apr;59:81-89
Domestic animal endocrinology 2017 Apr;59:81-89
No comments: Submit comment
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western Blot using AKR1C3 Polyclonal Antibody (Product # PA5-28065). Sample (30 µg of whole cell lysate). Lane A: HepG2. 10% SDS PAGE. AKR1C3 Polyclonal Antibody (Product # PA5-28065) diluted at 1:1,000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- AKR1C3 Polyclonal Antibody detects AKR1C3 protein by western blot analysis. A. 50 µg rat liver lysate/extract.10% SDS-PAGE. AKR1C3 Polyclonal Antibody (Product # PA5-28065) dilution: 1:2,000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunofluorescent analysis of AKR1C3 in paraformaldehyde-fixed HeLa cells using an AKR1C3 polyclonal antibody (Product # PA5-28065) at a 1:200 dilution.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunoprecipitation of AKR1C3 was performed in HepG2 whole cell extracts using 5 µg of AKR1C3 Polyclonal Antibody (Product # PA5-28065). Samples were transferred to a membrane and probed with AKR1C3 Polyclonal Antibody as a primary antibody and an HRP-conjugated anti-Rabbit IgG was used as a secondary antibody.
