PA5-51517

antibody from Invitrogen Antibodies

Targeting: WDHD1 AND-1, CHTF4, CTF4

Western blot (Supportive data in Antibodypedia)

Immunocytochemistry (Supportive data in Antibodypedia)

Immunohistochemistry (Supportive data in Antibodypedia)

Antibody Data

Product number

PA5-51517

Provider

Invitrogen Antibodies

Product name

WDHD1 Polyclonal Antibody

Antibody type

Polyclonal

Antigen

Recombinant full-length protein

Description

Immunogen sequence: QTLNIVTWSP CGQYLAAGSI NGLIIVWNVE TKDCMERVKH EKGYAICGLA WHPTCGRISY TDAEGNLGLL ENVCDPSGKT SSSKVSSRVE KDYNDLFDGD DMSNAGDFLN DNAVE Highest antigen sequence identity to the following orthologs: Mouse - 90%, Rat - 86%.

Reactivity

Human, Mouse, Rat

Host

Rabbit

Isotype

IgG

Vial size

100 µL

Concentration

0.1 mg/mL

Storage

Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

Western blot

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Western blot analysis of WDHD1 in Lane 1: Marker (kDa) 219, 112, 85, 49, 32, 25, 18; Lane 2: Human cell line RT-4; Lane 3: Human cell line U-251MG sp; Lane 4: Human cell line A-431. Samples were probed using a WDHD1 Polyclonal Antibody (Product # PA5-51517).

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Western blot analysis of WDHD1 in human cell line RH-30 using a WDHD1 Polyclonal Antibody (Product # PA5-51517).

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Western blot analysis of WDHD1 in mouse cell line NIH-3T3 and rat cell line NBT-II using a WDHD1 Polyclonal Antibody (Product # PA5-51517).

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Western blot analysis of WDHD1 in mouse cell line NIH-3T3 and rat cell line NBT-II using a WDHD1 Polyclonal Antibody (Product # PA5-51517).

Immunocytochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunofluorescent staining of WDHD1 in human cell line U-251 MG shows positivity in nucleus but excluded from the nucleoli. Samples were probed using a WDHD1 Polyclonal Antibody (Product # PA5-51517).

Immunohistochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemical staining of WDHD1 in human testis and liver tissues using WDHD1 Polyclonal Antibody (Product # PA5-51517). Corresponding WDHD1 RNA-seq data are presented for the same tissues.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemical staining of WDHD1 in human testis using WDHD1 Polyclonal Antibody (Product # PA5-51517) shows nuclear positivity in cells in seminiferous ducts.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemical staining of WDHD1 in human lymph node using WDHD1 Polyclonal Antibody (Product # PA5-51517) shows strong nuclear positivity in germinal center cells.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemical staining of WDHD1 in human rectum using WDHD1 Polyclonal Antibody (Product # PA5-51517) shows strong nuclear positivity in glandular cells.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemical staining of WDHD1 in human liver using WDHD1 Polyclonal Antibody (Product # PA5-51517) shows no positivity in hepatocytes.