Antibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Western blot [1]
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Validation data
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- Product number
- 630301 - Provider product page
- Provider
- BioLegend
- Proper citation
- BioLegend Cat#630301, RRID:AB_2215084
- Product name
- Purified anti-AND-1 (WDHD1)
- Antibody type
- Monoclonal
- Antigen
- Recombinant (partial), C-terminal
- Description
- The 20G10 monoclonal antibody recognizes both human and mouse AND-1 (WDHD1) also known as WD repeat and HMG box DNA binding protein 1, acidic nucleoplasmic DNA-binding protein 1. AND-1 (WDHD1) is a nuclear and nucleolar protein that contains multiple WD40 repeats, and one HMG region. AND-1 (WDHD1) has a predicted molecular weight of 126 kD and can form homodimers. WDHD1 is expressed in the brain, epidermis, liver, and stomach. During mitosis, AND-1 (WDHD1) is transiently expressed in nucleoplasm. AND-1 (WDHD1) can bind to DNA with high affinity and may be involved in protein-protein interactions. The role of AND-1 (WDHD1) is thought to be chromatin assembly, transcription and DNA replication. AND-1 (WDHD1) has been reported to be phosphorylated on S1041. The 20G10 antibody has been shown to be useful for Western blotting.
- Reactivity
- Human, Mouse
- Host
- Mouse
- Conjugate
- Unconjugated
- Isotype
- IgG
- Vial size
- 25 µg
- Concentration
- 0.5 mg/ml
- Storage
- The antibody solution should be stored undiluted at 4°C.
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Supportive validation
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- BioLegend (provider)
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- Experimental details
- Formalin-fixed paraffin-embedded human kidney tissue was stained with 20G10 at 15 µg/ml and developed with an alkaline phosphatase chromogen substrate (red color). Tissue was counterstained with H&E (blue/pink). Magnification, 40X.
- Conjugate
- Unconjugated