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LearnMAB21651-100
antibody from R&D Systems
Targeting: CXCR4
CD184, D2S201E, fusin, HM89, HSY3RR, LESTR, NPY3R, NPYR, NPYY3R
ImmunocytochemistryAntibody data
- Antibody Data
- Antigen structure
- References [25]
- Comments [0]
- Validations
- Immunocytochemistry [2]
- Immunohistochemistry [1]
- Flow cytometry [1]
- Blocking/Neutralizing [1]
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- Product number
- MAB21651-100 - Provider product page
- Provider
- R&D Systems
- Product name
- Mouse CXCR4 Antibody
- Antibody type
- Monoclonal
- Description
- Protein A or G purified from hybridoma culture supernatant. Detects mouse CXCR4 transfectants. Does not stain irrelevant transfectants.
- Reactivity
- Mouse
- Host
- Rat
- Conjugate
- Unconjugated
- Antigen sequence
P70658- Isotype
- IgG
- Antibody clone number
- 247506
- Vial size
- 100 ug
- Concentration
- LYOPH
- Storage
- Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Submitted references Secreted nuclear protein DEK regulates hematopoiesis through CXCR2 signaling.
The Chemokine Receptor CXCR4 Mediates Recruitment of CD11c+ Conventional Dendritic Cells Into the Inflamed Murine Cornea.
Proinflammatory CXCL12-CXCR4/CXCR7 Signaling Axis Drives Myc-Induced Prostate Cancer in Obese Mice.
Loss of Ranbp2 in motoneurons causes disruption of nucleocytoplasmic and chemokine signaling, proteostasis of hnRNPH3 and Mmp28, and development of amyotrophic lateral sclerosis-like syndromes.
CXCR4 signaling in macrophages contributes to periodontal mechanical hypersensitivity in Porphyromonas gingivalis-induced periodontitis in mice.
Natural amines inhibit activation of human plasmacytoid dendritic cells through CXCR4 engagement.
CXCR4 signaling contributes to alveolar bone resorption in Porphyromonas gingivalis-induced periodontitis in mice.
CXCL12 enhances angiogenesis through CXCR7 activation in human umbilical vein endothelial cells.
A gonogenic stimulated transition of mouse embryonic stem cells with enhanced control of diverse differentiation pathways.
NKG2D ligand overexpression in lupus nephritis correlates with increased NK cell activity and differentiation in kidneys but not in the periphery.
CXCR7 Mediates Neural Progenitor Cells Migration to CXCL12 Independent of CXCR4.
Nuclear factor kappa-B signaling is integral to ocular neovascularization in ischemia-independent microenvironment.
An important role of the SDF-1/CXCR4 axis in chronic skin inflammation.
Protein z exerts pro-angiogenic effects and upregulates CXCR4.
Systemic neutralization of IL-17A significantly reduces breast cancer associated metastasis in arthritic mice by reducing CXCL12/SDF-1 expression in the metastatic niches.
Mechanisms of adhesion and subsequent actions of a haematopoietic stem cell line, HPC-7, in the injured murine intestinal microcirculation in vivo.
CXCL12-CXCR4 signaling is required for the maintenance of mouse spermatogonial stem cells.
The role of SDF-1-CXCR4/CXCR7 axis in the therapeutic effects of hypoxia-preconditioned mesenchymal stem cells for renal ischemia/reperfusion injury.
Influence of hypoxia-inducible factor 1α on dendritic cell differentiation and migration.
MicroRNA126 contributes to granulocyte colony-stimulating factor-induced hematopoietic progenitor cell mobilization by reducing the expression of vascular cell adhesion molecule 1.
IL-1α and IL-1β recruit different myeloid cells and promote different stages of sterile inflammation.
Paracrine modulation of CXCR4 by IGF-1 and VEGF: implications for choroidal neovascularization.
Microarray analysis of retinal endothelial tip cells identifies CXCR4 as a mediator of tip cell morphology and branching.
Regulation of CXCR4 by the Notch ligand delta-like 4 in endothelial cells.
The angiogenic response is dictated by beta3 integrin on bone marrow-derived cells.
Capitano ML, Mor-Vaknin N, Saha AK, Cooper S, Legendre M, Guo H, Contreras-Galindo R, Kappes F, Sartor MA, Lee CT, Huang X, Markovitz DM, Broxmeyer HE
The Journal of clinical investigation 2019 May 20;129(6):2555-2570
The Journal of clinical investigation 2019 May 20;129(6):2555-2570
The Chemokine Receptor CXCR4 Mediates Recruitment of CD11c+ Conventional Dendritic Cells Into the Inflamed Murine Cornea.
Lopez MJ, Seyed-Razavi Y, Jamali A, Harris DL, Hamrah P
Investigative ophthalmology & visual science 2018 Nov 1;59(13):5671-5681
Investigative ophthalmology & visual science 2018 Nov 1;59(13):5671-5681
Proinflammatory CXCL12-CXCR4/CXCR7 Signaling Axis Drives Myc-Induced Prostate Cancer in Obese Mice.
Saha A, Ahn S, Blando J, Su F, Kolonin MG, DiGiovanni J
Cancer research 2017 Sep 15;77(18):5158-5168
Cancer research 2017 Sep 15;77(18):5158-5168
Loss of Ranbp2 in motoneurons causes disruption of nucleocytoplasmic and chemokine signaling, proteostasis of hnRNPH3 and Mmp28, and development of amyotrophic lateral sclerosis-like syndromes.
Cho KI, Yoon D, Qiu S, Danziger Z, Grill WM, Wetsel WC, Ferreira PA
Disease models & mechanisms 2017 May 1;10(5):559-579
Disease models & mechanisms 2017 May 1;10(5):559-579
CXCR4 signaling in macrophages contributes to periodontal mechanical hypersensitivity in Porphyromonas gingivalis-induced periodontitis in mice.
Nagashima H, Shinoda M, Honda K, Kamio N, Watanabe M, Suzuki T, Sugano N, Sato S, Iwata K
Molecular pain 2017 Jan;13:1744806916689269
Molecular pain 2017 Jan;13:1744806916689269
Natural amines inhibit activation of human plasmacytoid dendritic cells through CXCR4 engagement.
Smith N, Pietrancosta N, Davidson S, Dutrieux J, Chauveau L, Cutolo P, Dy M, Scott-Algara D, Manoury B, Zirafi O, McCort-Tranchepain I, Durroux T, Bachelerie F, Schwartz O, Münch J, Wack A, Nisole S, Herbeuval JP
Nature communications 2017 Feb 9;8:14253
Nature communications 2017 Feb 9;8:14253
CXCR4 signaling contributes to alveolar bone resorption in Porphyromonas gingivalis-induced periodontitis in mice.
Nagashima H, Shinoda M, Honda K, Kamio N, Hasuike A, Sugano N, Arai Y, Sato S, Iwata K
Journal of oral science 2017 Dec 27;59(4):571-577
Journal of oral science 2017 Dec 27;59(4):571-577
CXCL12 enhances angiogenesis through CXCR7 activation in human umbilical vein endothelial cells.
Zhang M, Qiu L, Zhang Y, Xu D, Zheng JC, Jiang L
Scientific reports 2017 Aug 15;7(1):8289
Scientific reports 2017 Aug 15;7(1):8289
A gonogenic stimulated transition of mouse embryonic stem cells with enhanced control of diverse differentiation pathways.
Moshfegh C, Aires L, Kisielow M, Vogel V
Scientific reports 2016 May 9;6:25104
Scientific reports 2016 May 9;6:25104
NKG2D ligand overexpression in lupus nephritis correlates with increased NK cell activity and differentiation in kidneys but not in the periphery.
Spada R, Rojas JM, Pérez-Yagüe S, Mulens V, Cannata-Ortiz P, Bragado R, Barber DF
Journal of leukocyte biology 2015 Mar;97(3):583-98
Journal of leukocyte biology 2015 Mar;97(3):583-98
CXCR7 Mediates Neural Progenitor Cells Migration to CXCL12 Independent of CXCR4.
Chen Q, Zhang M, Li Y, Xu D, Wang Y, Song A, Zhu B, Huang Y, Zheng JC
Stem cells (Dayton, Ohio) 2015 Aug;33(8):2574-85
Stem cells (Dayton, Ohio) 2015 Aug;33(8):2574-85
Nuclear factor kappa-B signaling is integral to ocular neovascularization in ischemia-independent microenvironment.
DeNiro M, Al-Mohanna FA
PloS one 2014;9(7):e101602
PloS one 2014;9(7):e101602
An important role of the SDF-1/CXCR4 axis in chronic skin inflammation.
Zgraggen S, Huggenberger R, Kerl K, Detmar M
PloS one 2014;9(4):e93665
PloS one 2014;9(4):e93665
Protein z exerts pro-angiogenic effects and upregulates CXCR4.
Butschkau A, Wagner NM, Genz B, Vollmar B
PloS one 2014;9(12):e113554
PloS one 2014;9(12):e113554
Systemic neutralization of IL-17A significantly reduces breast cancer associated metastasis in arthritic mice by reducing CXCL12/SDF-1 expression in the metastatic niches.
Roy LD, Sahraei M, Schettini JL, Gruber HE, Besmer DM, Mukherjee P
BMC cancer 2014 Mar 27;14:225
BMC cancer 2014 Mar 27;14:225
Mechanisms of adhesion and subsequent actions of a haematopoietic stem cell line, HPC-7, in the injured murine intestinal microcirculation in vivo.
Kavanagh DP, Yemm AI, Zhao Y, Frampton J, Kalia N
PloS one 2013;8(3):e59150
PloS one 2013;8(3):e59150
CXCL12-CXCR4 signaling is required for the maintenance of mouse spermatogonial stem cells.
Yang QE, Kim D, Kaucher A, Oatley MJ, Oatley JM
Journal of cell science 2013 Feb 15;126(Pt 4):1009-20
Journal of cell science 2013 Feb 15;126(Pt 4):1009-20
The role of SDF-1-CXCR4/CXCR7 axis in the therapeutic effects of hypoxia-preconditioned mesenchymal stem cells for renal ischemia/reperfusion injury.
Liu H, Liu S, Li Y, Wang X, Xue W, Ge G, Luo X
PloS one 2012;7(4):e34608
PloS one 2012;7(4):e34608
Influence of hypoxia-inducible factor 1α on dendritic cell differentiation and migration.
Köhler T, Reizis B, Johnson RS, Weighardt H, Förster I
European journal of immunology 2012 May;42(5):1226-36
European journal of immunology 2012 May;42(5):1226-36
MicroRNA126 contributes to granulocyte colony-stimulating factor-induced hematopoietic progenitor cell mobilization by reducing the expression of vascular cell adhesion molecule 1.
Salvucci O, Jiang K, Gasperini P, Maric D, Zhu J, Sakakibara S, Espigol-Frigole G, Wang S, Tosato G
Haematologica 2012 Jun;97(6):818-26
Haematologica 2012 Jun;97(6):818-26
IL-1α and IL-1β recruit different myeloid cells and promote different stages of sterile inflammation.
Rider P, Carmi Y, Guttman O, Braiman A, Cohen I, Voronov E, White MR, Dinarello CA, Apte RN
Journal of immunology (Baltimore, Md. : 1950) 2011 Nov 1;187(9):4835-43
Journal of immunology (Baltimore, Md. : 1950) 2011 Nov 1;187(9):4835-43
Paracrine modulation of CXCR4 by IGF-1 and VEGF: implications for choroidal neovascularization.
Sengupta N, Afzal A, Caballero S, Chang KH, Shaw LC, Pang JJ, Bond VC, Bhutto I, Baba T, Lutty GA, Grant MB
Investigative ophthalmology & visual science 2010 May;51(5):2697-704
Investigative ophthalmology & visual science 2010 May;51(5):2697-704
Microarray analysis of retinal endothelial tip cells identifies CXCR4 as a mediator of tip cell morphology and branching.
Strasser GA, Kaminker JS, Tessier-Lavigne M
Blood 2010 Jun 17;115(24):5102-10
Blood 2010 Jun 17;115(24):5102-10
Regulation of CXCR4 by the Notch ligand delta-like 4 in endothelial cells.
Williams CK, Segarra M, Sierra Mde L, Sainson RC, Tosato G, Harris AL
Cancer research 2008 Mar 15;68(6):1889-95
Cancer research 2008 Mar 15;68(6):1889-95
The angiogenic response is dictated by beta3 integrin on bone marrow-derived cells.
Feng W, McCabe NP, Mahabeleshwar GH, Somanath PR, Phillips DR, Byzova TV
The Journal of cell biology 2008 Dec 15;183(6):1145-57
The Journal of cell biology 2008 Dec 15;183(6):1145-57
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Supportive validation
- Submitted by
- R&D Systems (provider)
- Main image
- Experimental details
- CXCR4 in Mouse Neural Progenitor Cells. CXCR4 was detected in immersion fixed mouse neural progenitor cells using 10 µg/mL Rat Anti-Mouse CXCR4 Monoclonal Antibody (Catalog # MAB21651) for 3 hours at room temperature. Cells were stained with the NorthernLights™ 557-conjugated Anti-Rat IgG Secondary Antibody (red; Catalog # NL013) and counterstained with DAPI (blue). View our protocol for Fluorescent ICC Staining of Cells on Coverslips.
- Submitted by
- R&D Systems (provider)
- Main image
- Experimental details
- CXCR4 in Mouse Splenocytes. CXCR4 was detected in immersion fixed mouse splenocytes using 10 µg/mL Rat Anti-Mouse CXCR4 Monoclonal Antibody (Catalog # MAB21651) for 3 hours at room temperature. Cells were stained with the NorthernLights™ 557-conjugated Anti-Rat IgG Secondary Antibody (red; Catalog # NL013) and counterstained with DAPI (blue). View our protocol for Fluorescent ICC Staining of Non-adherent Cells.
Supportive validation
- Submitted by
- R&D Systems (provider)
- Main image
- Experimental details
- CXCR4 in Mouse Spleen. CXCR4 was detected in immersion fixed frozen sections of mouse spleen using Rat Anti-Mouse CXCR4 Monoclonal Antibody (Catalog # MAB21651) at 15 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Rat HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS017) and counterstained with hematoxylin (blue). Lower panel shows a lack of labeling if primary antibodies are omitted and tissue is stained only with secondary antibody followed by incubation with detection reagents. View our protocol for Chromogenic IHC Staining of Frozen Tissue Sections.
Supportive validation
- Submitted by
- R&D Systems (provider)
- Main image
- Experimental details
- Detection of CXCR4 in Mouse Thymocytes by Flow Cytometry. Mouse thymocytes were stained with (A) Rat Anti-Mouse CXCR4 Monoclonal Antibody (Catalog # MAB21651) or (B) Rat IgG2B isotype control antibody (Catalog # MAB0061) followed by anti-Rat IgG PE-conjugated Secondary Antibody (Catalog # F0105B) and Rat anti-Mouse CD8 alpha APC-conjugated Monoclonal Antibody (Catalog # FAB116A). View our protocol for Staining Membrane-associated Proteins.
Supportive validation
- Submitted by
- R&D Systems (provider)
- Main image
- Experimental details
- Chemotaxis Induced by CXCL12/SDF-1 alpha and Neutralization by Mouse CXCR4 Antibody. Recombinant Mouse CXCL12/SDF-1 alpha (Catalog # 460-SD) chemo-attracts the BaF3 mouse pro-B cell line transfected with mouse CXCR4 in a dose-dependent manner (orange line). The amount of cells that migrated through to the lower chemotaxis chamber was measured by Resazurin (Catalog # AR002). Chemotaxis elicited by Recombinant Mouse CXCL12/ SDF-1 alpha (10 ng/mL) is neutral-ized (green line) by increasing concentrations of Rat Anti-Mouse CXCR4 Monoclonal Antibody (Catalog # MAB21651). The ND50 is typically 8-40 µg/mL.
