MHCXCR404

antibody from Invitrogen Antibodies

Targeting: CXCR4 CD184, D2S201E, fusin, HM89, HSY3RR, LESTR, NPY3R, NPYR, NPYY3R

Flow cytometry

Antibody data

Submit

Validation data

Product number: MHCXCR404 - Provider product page
Provider: Invitrogen Antibodies
Product name: CXCR4 Monoclonal Antibody (12G5), PE
Antibody type: Monoclonal
Antigen: Other
Description: R-phycoerythrin (PE) is a stable and highly soluble phycobiliprotein which provides maximal absorbance and fluorescence without susceptibility to internal or external fluorescence quenching, thus providing an exceptional quantum yields and molar extinction coefficients.
Reactivity: Human
Host: Mouse
Conjugate: Yellow dye
Isotype: IgG
Antibody clone number: 12G5
Vial size: 500 µL
Storage: 4° C, store in dark

CXCR4 protein structure - MHCXCR404 shown in red.

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Figure 2 Hepatocyte specification yields luciferase expression in cells derived from CYP1A1 reporter iPSCs. (a) iPSCs were differentiated towards CXCR4+/C-KIT+ definitive endoderm (day 5) followed by FOXA1+/AAT+ hepatic progenitors (day 14) that grew in number and were the majority of the culture by day 25. (b) Micrographs show homogenous morphology of definitive endoderm cultures (day 5), but by day 14, hepatic-like cells begin to emerge (denoted by black arrowheads) and are observed more frequently by day 25. (c) Concomitant with hepatic specification, luciferase levels significantly increase ( N = 3, * P < 0.0005, Student's t -test).
Conjugate: Yellow dye

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Figure 3 Quality check via flow cytometry Differentiation efficiencies at definitive endoderm (DE, d3), pancreatic endoderm (PE, d9), and pancreatic progenitor (PP, d13) stage are measured by flow cytometry. Our quality standards are 95% CXCR4 + /KIT + cells at DE stage, 90% PDX1 + cells at PE stage, and 60% PDX1 + /NKX6-1 + cells at PP stage.
Conjugate: Yellow dye

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Figure 4 DSC2 and SLC22A1 KO lines show an impaired differentiation into definitive endoderm. ( A ) Newly generated KO cell lines were differentiated towards DE stage with either the standard induction protocol (left), or with only 10% or 5% (right) of standard activin A concentration. ( B ) Differentiation efficiency was analysed by flow cytometry of DE markers CXCR4 and KIT, as shown in a representative FACS plot. ( C ) From cells generated under optimal culture conditions, RNA samples were taken and analysed via qPCR for expression levels of DE markers CXCR4 , FOXA2 , SOX17, and GATA6 . Experiments with standard culture conditions were performed 3 times, using 2 different clones per genotype in technical duplicates (dots represent means of duplicates). Experiments with reduced levels of activin A were performed once with 2 different clones per genotype and in duplicates (dots represent means of these duplicates). Gene expression was first normalized to housekeeping gene HMBS and then normalized to WT gene expression. Mann-Whitney test was used for analysis; error bars represent mean +- SD, *** p < 0.001, ** p < 0.01, * p < 0.05.
Conjugate: Yellow dye

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Fig. 1 CXCR4 expression analysis in response to DNA damage. a Gene expression changes after BrdU treatment in HeLa cells. Expression pattern of genes as indicated after 48 h of BrdU treatment (100 muM) by microarray analysis. Data extracted from the microarray experiment is reported in Supplementary Fig. S1a ( n = 2). The numbers indicate fold change in gene expression during BrdU treatment wrt Control (normalized as 1). b CXCR4 gene expression analysis after DNA damage. HeLa and A549 cells were treated for 48 h with 100 muM BrdU followed by qRT-PCR analysis of CXCR4 expression. The values were normalized to beta-actin expression and then wrt control cells to calculate fold changes. Results shown are mean +- s.e.m. * p 3). d Gene expression analysis during replicative exhaustion mediated DNA damage and senescence. Expression analysis of CXCR4 (left) and DDR genes P21 and P53 (right) in early (20 PDL) and late (40 PDL) passage MRC5 cells by qRT-PCR. The values were normalized to beta-actin expression and then wrt control cells to calculate fold changes ( n
Conjugate: Yellow dye