13-9999-80
antibody from Invitrogen Antibodies
Targeting: CXCR4
CD184, D2S201E, fusin, HM89, HSY3RR, LESTR, NPY3R, NPYR, NPYY3R
Antibody data
- Antibody Data
- Antigen structure
- References [7]
- Comments [0]
- Validations
- Flow cytometry [1]
Submit
Validation data
Reference
Comment
Report error
- Product number
- 13-9999-80 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- Anti-CD184 (CXCR4) Monoclonal Antibody (12G5), Biotin, eBioscience™
- Antibody type
- Monoclonal
- Antigen
- Other
- Description
- Description: The 12G5 monoclonal antibody reacts with human CD184 (CXCR4), also termed Fusin, LESTR, or HUMSTR. CD184 is a member of the G-protein-coupled chemokine receptor family with seven membrane-spanning domains, and functions as a coreceptor for X4 HIV-1 entry into CD4+ cells. CD184 is expressed predominantly on naive T cell subsets of peripheral blood and is rapidly upregulated by PHA and IL-2 stimulation. The 12G5 antibody shows partial inhibition of chemotaxis and calcium influx induced by SDF-1 (the natural ligand of CD184), blocks CD4-independent HIV-2 infection, and blocks CD4-dependent infection by some T-tropic HIV-1 isolates. Applications Reported: The 12G5 antibody has been reported for use in flow cytometric analysis. Applications Tested: This 12G5 antibody has been tested by flow cytometric analysis of normal human peripheral blood cells. This can be used at less than or equal to 0.5 µg per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest. Filtration: 0.2 µm post-manufacturing filtered.
- Reactivity
- Human
- Host
- Mouse
- Conjugate
- Biotin
- Isotype
- IgG
- Antibody clone number
- 12G5
- Vial size
- 25 µg
- Concentration
- 0.5 mg/mL
- Storage
- 4° C, store in dark, DO NOT FREEZE!
Submitted references ST8SIA4-Dependent Polysialylation is Part of a Developmental Program Required for Germ Layer Formation from Human Pluripotent Stem Cells.
Antibody epitopes on g protein-coupled receptors mapped with genetically encoded photoactivatable cross-linkers.
Human cytomegalovirus infection inhibits CXCL12- mediated migration and invasion of human extravillous cytotrophoblasts.
Osteopontin enhances the expression and activity of MMP-2 via the SDF-1/CXCR4 axis in hepatocellular carcinoma cell lines.
The HIV coreceptors CXCR4 and CCR5 are differentially expressed and regulated on human T lymphocytes.
The HIV coreceptors CXCR4 and CCR5 are differentially expressed and regulated on human T lymphocytes.
Inhibition of human immunodeficiency virus fusion by a monoclonal antibody to a coreceptor (CXCR4) is both cell type and virus strain dependent.
Berger RP, Sun YH, Kulik M, Lee JK, Nairn AV, Moremen KW, Pierce M, Dalton S
Stem cells (Dayton, Ohio) 2016 Jul;34(7):1742-52
Stem cells (Dayton, Ohio) 2016 Jul;34(7):1742-52
Antibody epitopes on g protein-coupled receptors mapped with genetically encoded photoactivatable cross-linkers.
Ray-Saha S, Huber T, Sakmar TP
Biochemistry 2014 Mar 4;53(8):1302-10
Biochemistry 2014 Mar 4;53(8):1302-10
Human cytomegalovirus infection inhibits CXCL12- mediated migration and invasion of human extravillous cytotrophoblasts.
Warner JA, Zwezdaryk KJ, Day B, Sullivan DE, Pridjian G, Morris CA
Virology journal 2012 Nov 1;9:255
Virology journal 2012 Nov 1;9:255
Osteopontin enhances the expression and activity of MMP-2 via the SDF-1/CXCR4 axis in hepatocellular carcinoma cell lines.
Zhang R, Pan X, Huang Z, Weber GF, Zhang G
PloS one 2011;6(8):e23831
PloS one 2011;6(8):e23831
The HIV coreceptors CXCR4 and CCR5 are differentially expressed and regulated on human T lymphocytes.
Bleul CC, Wu L, Hoxie JA, Springer TA, Mackay CR
Proceedings of the National Academy of Sciences of the United States of America 1997 Mar 4;94(5):1925-30
Proceedings of the National Academy of Sciences of the United States of America 1997 Mar 4;94(5):1925-30
The HIV coreceptors CXCR4 and CCR5 are differentially expressed and regulated on human T lymphocytes.
Bleul CC, Wu L, Hoxie JA, Springer TA, Mackay CR
Proceedings of the National Academy of Sciences of the United States of America 1997 Mar 4;94(5):1925-30
Proceedings of the National Academy of Sciences of the United States of America 1997 Mar 4;94(5):1925-30
Inhibition of human immunodeficiency virus fusion by a monoclonal antibody to a coreceptor (CXCR4) is both cell type and virus strain dependent.
McKnight A, Wilkinson D, Simmons G, Talbot S, Picard L, Ahuja M, Marsh M, Hoxie JA, Clapham PR
Journal of virology 1997 Feb;71(2):1692-6
Journal of virology 1997 Feb;71(2):1692-6
No comments: Submit comment
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Staining of normal human peripheral blood cells with staining buffer (autofluorescence) (open histogram) or Anti-Human CD184 (CXCR4) PE-Cyanine5 (filled histogram). Cells in the lymphocyte gate were used for analysis.
- Conjugate
- Biotin