25-9999-41
antibody from Invitrogen Antibodies
Targeting: CXCR4
CD184, D2S201E, fusin, HM89, HSY3RR, LESTR, NPY3R, NPYR, NPYY3R
Antibody data
- Antibody Data
- Antigen structure
- References [11]
- Comments [0]
- Validations
- Flow cytometry [1]
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- Product number
- 25-9999-41 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- Anti-CD184 (CXCR4) Monoclonal Antibody (12G5), PE-Cyanine7, eBioscience™
- Antibody type
- Monoclonal
- Antigen
- Other
- Description
- Description: The 12G5 monoclonal antibody reacts with human CD184 (CXCR4), also termed Fusin, LESTR, or HUMSTR. CD184 is a member of the G-protein-coupled chemokine receptor family with seven membrane-spanning domains, and functions as a coreceptor for X4 HIV-1 entry into CD4+ cells. CD184 is expressed predominantly on naive T cell subsets of peripheral blood and is rapidly upregulated by PHA and IL-2 stimulation. The 12G5 antibody shows partial inhibition of chemotaxis and calcium influx induced by SDF-1 (the natural ligand of CD184), blocks CD4-independent HIV-2 infection, and blocks CD4-dependent infection by some T-tropic HIV-1 isolates. Applications Reported: This 12G5 antibody has been reported for use in flow cytometric analysis. Applications Tested: This 12G5 antibody has been pre-titrated and tested by flow cytometric analysis of normal human peripheral blood cells. This can be used at 5 µL (0.25 µg) per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. Light sensitivity: This tandem dye is sensitive photo-induced oxidation. Please protect this vial and stained samples from light. Fixation: Samples can be stored in IC Fixation Buffer (cat. 00-8222) (100 µL cell sample + 100 µL IC Fixation Buffer) or 1-step Fix/Lyse Solution (cat. 00-5333) for up to 3 days in the dark at 4°C with minimal impact on brightness and FRET efficiency/compensation. Some generalizations regarding fluorophore performance after fixation can be made, but clone specific performance should be determined empirically. Excitation: 488-561 nm; Emission: 775 nm; Laser: Blue Laser, Green Laser, Yellow-Green Laser. Filtration: 0.2 µm post-manufacturing filtered.
- Reactivity
- Human
- Host
- Mouse
- Isotype
- IgG
- Antibody clone number
- 12G5
- Vial size
- 25 Tests
- Concentration
- 5 µL/Test
- Storage
- 4° C, store in dark, DO NOT FREEZE!
Submitted references The CXCR4 antagonist plerixafor (AMD3100) promotes proliferation of Ewing sarcoma cell lines in vitro and activates receptor tyrosine kinase signaling.
Sex Differences in Circulating Progenitor Cells.
CRISPR/Cas9-based genetic correction for recessive dystrophic epidermolysis bullosa.
Age and Human Regenerative Capacity Impact of Cardiovascular Risk Factors.
ST8SIA4-Dependent Polysialylation is Part of a Developmental Program Required for Germ Layer Formation from Human Pluripotent Stem Cells.
Circulating Progenitor Cells is Linked to Cognitive Decline in Healthy Adults.
Circulating CD34+ progenitor cells and risk of mortality in a population with coronary artery disease.
Plerixafor as a chemosensitizing agent in pediatric acute lymphoblastic leukemia: efficacy and potential mechanisms of resistance to CXCR4 inhibition.
Tumor-derived exosomes promote tumor progression and T-cell dysfunction through the regulation of enriched exosomal microRNAs in human nasopharyngeal carcinoma.
Effect of progenitor cell mobilization with granulocyte-macrophage colony-stimulating factor in patients with peripheral artery disease: a randomized clinical trial.
Elevated circulating angiogenic progenitors and white blood cells are associated with hypoxia-inducible angiogenic growth factors in children with sickle cell disease.
Berning P, Schaefer C, Clemens D, Korsching E, Dirksen U, Potratz J
Cell communication and signaling : CCS 2018 May 18;16(1):21
Cell communication and signaling : CCS 2018 May 18;16(1):21
Sex Differences in Circulating Progenitor Cells.
Topel ML, Hayek SS, Ko YA, Sandesara PB, Samman Tahhan A, Hesaroieh I, Mahar E, Martin GS, Waller EK, Quyyumi AA
Journal of the American Heart Association 2017 Oct 3;6(10)
Journal of the American Heart Association 2017 Oct 3;6(10)
CRISPR/Cas9-based genetic correction for recessive dystrophic epidermolysis bullosa.
Webber BR, Osborn MJ, McElroy AN, Twaroski K, Lonetree CL, DeFeo AP, Xia L, Eide C, Lees CJ, McElmurry RT, Riddle MJ, Kim CJ, Patel DD, Blazar BR, Tolar J
NPJ Regenerative medicine 2016;1
NPJ Regenerative medicine 2016;1
Age and Human Regenerative Capacity Impact of Cardiovascular Risk Factors.
Al Mheid I, Hayek SS, Ko YA, Akbik F, Li Q, Ghasemzadeh N, Martin GS, Long Q, Hammadah M, Maziar Zafari A, Vaccarino V, Waller EK, Quyyumi AA
Circulation research 2016 Sep 16;119(7):801-9
Circulation research 2016 Sep 16;119(7):801-9
ST8SIA4-Dependent Polysialylation is Part of a Developmental Program Required for Germ Layer Formation from Human Pluripotent Stem Cells.
Berger RP, Sun YH, Kulik M, Lee JK, Nairn AV, Moremen KW, Pierce M, Dalton S
Stem cells (Dayton, Ohio) 2016 Jul;34(7):1742-52
Stem cells (Dayton, Ohio) 2016 Jul;34(7):1742-52
Circulating Progenitor Cells is Linked to Cognitive Decline in Healthy Adults.
Hajjar I, Goldstein FC, Waller EK, Moss LD, Quyyumi A
The American journal of the medical sciences 2016 Feb;351(2):147-52
The American journal of the medical sciences 2016 Feb;351(2):147-52
Circulating CD34+ progenitor cells and risk of mortality in a population with coronary artery disease.
Patel RS, Li Q, Ghasemzadeh N, Eapen DJ, Moss LD, Janjua AU, Manocha P, Kassem HA, Veledar E, Samady H, Taylor WR, Zafari AM, Sperling L, Vaccarino V, Waller EK, Quyyumi AA
Circulation research 2015 Jan 16;116(2):289-297
Circulation research 2015 Jan 16;116(2):289-297
Plerixafor as a chemosensitizing agent in pediatric acute lymphoblastic leukemia: efficacy and potential mechanisms of resistance to CXCR4 inhibition.
Sison EA, Magoon D, Li L, Annesley CE, Rau RE, Small D, Brown P
Oncotarget 2014 Oct 15;5(19):8947-58
Oncotarget 2014 Oct 15;5(19):8947-58
Tumor-derived exosomes promote tumor progression and T-cell dysfunction through the regulation of enriched exosomal microRNAs in human nasopharyngeal carcinoma.
Ye SB, Li ZL, Luo DH, Huang BJ, Chen YS, Zhang XS, Cui J, Zeng YX, Li J
Oncotarget 2014 Jul 30;5(14):5439-52
Oncotarget 2014 Jul 30;5(14):5439-52
Effect of progenitor cell mobilization with granulocyte-macrophage colony-stimulating factor in patients with peripheral artery disease: a randomized clinical trial.
Poole J, Mavromatis K, Binongo JN, Khan A, Li Q, Khayata M, Rocco E, Topel M, Zhang X, Brown C, Corriere MA, Murrow J, Sher S, Clement S, Ashraf K, Rashed A, Kabbany T, Neuman R, Morris A, Ali A, Hayek S, Oshinski J, Yoon YS, Waller EK, Quyyumi AA
JAMA 2013 Dec 25;310(24):2631-9
JAMA 2013 Dec 25;310(24):2631-9
Elevated circulating angiogenic progenitors and white blood cells are associated with hypoxia-inducible angiogenic growth factors in children with sickle cell disease.
Ofori-Acquah SF, Buchanan ID, Osunkwo I, Manlove-Simmons J, Lawal F, Quarshie A, Quyyumi AA, Gibbons GH, Gee BE
Anemia 2012;2012:156598
Anemia 2012;2012:156598
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Staining of normal human peripheral blood cells with Anti-Human CD19 FITC (Product # 11-0199-42) and Mouse IgG2a K Isotype Control PE-Cyanine7 (Product # 25-4724-81) (left) or Anti-Human CD184 (CXCR4) PE-Cyanine7 (right). Cells in the lymphocyte gate were used for analysis.