PA5-117898

antibody from Invitrogen Antibodies

Targeting: CXCR4 CD184, D2S201E, fusin, HM89, HSY3RR, LESTR, NPY3R, NPYR, NPYY3R

Western blot (Supportive data in Antibodypedia)

Immunoprecipitation (Data presented on provider website)

Other assay (Supportive data in Antibodypedia)

Antibody Data

Product number

PA5-117898

Provider

Invitrogen Antibodies

Product name

CXCR4 Polyclonal Antibody

Antibody type

Polyclonal

Antigen

Other

Reactivity

Human

Host

Rabbit

Isotype

IgG

Vial size

100 µL

Storage

-20° C, Avoid Freeze/Thaw Cycles

Western blot

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Western Blot using CXCR4 Polyclonal Antibody (Product # PA5-117898) at 1:500 dilution. Lane A: Hela Whole Cell Lysate, Lane B: HepG2 Whole Cell Lysate, Lane C: K562 Whole Cell Lysate. Lysates/proteins at 30 μg per lane. Secondary antibody: Goat Anti-Rabbit IgG (H+L)/HRP at 1:10,000 dilution. Developed using the ECL technique. Performed under reducing conditions. Predicted band size: 39 kDa. Observed band size: 46 kDa.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Western Blot using CXCR4 Polyclonal Antibody (Product # PA5-117898) at 1:500 dilution. Lane A: Hela Whole Cell Lysate, Lane B: HepG2 Whole Cell Lysate, Lane C: K562 Whole Cell Lysate. Lysates/proteins at 30 μg per lane. Secondary antibody: Goat Anti-Rabbit IgG (H+L)/HRP at 1:10,000 dilution. Developed using the ECL technique. Performed under reducing conditions. Predicted band size: 39 kDa. Observed band size: 46 kDa.

Other assay

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

CXCR4 Immunoprecipitation using: Lane A: 0.5 mg HepG2 Whole Cell Lysate 1 µL with CXCR4 Polyclonal Antibody (Product # PA5-117898) and 60 μg of Immunomagnetic beads Protein A/G. Primary antibody: CXCR4 Polyclonal Antibody, at 1:500 dilution. Secondary antibody: Clean-Blot IP Detection Reagent (HRP) at 1:1,000dilution. Developed using the ECL technique. Performed under reducing conditions. Predicted band size: 39 kDa. Observed band size: 45 kDa.