MA1-754

antibody from Invitrogen Antibodies

Targeting: PSEN2 AD3L, AD4, PS2, STM2

Western blot (Data presented on provider website)

ELISA (Recommended by provider)

Immunocytochemistry (Supportive data in Antibodypedia)

Immunoprecipitation (Recommended by provider)

Immunohistochemistry (Data presented on provider website)

Antibody Data

Product number

MA1-754

Provider

Invitrogen Antibodies

Product name

PSEN2 Monoclonal Antibody (APS 26)

Antibody type

Monoclonal

Antigen

Synthetic peptide

Description

MA1-754 detects presenilin 2 protein (PS2) from rat, mouse, human, and nonhuman primate samples. No cross-reactivity is seen with presenilin 1. MA1-754 has successfully been used in immuno-fluorescence, immunocytochemistry, Western blot and ELISA procedures. By Western blot, this antibody detects an ~20 kDa protein representing PS2 CT (C-terminal fragment) and the ~45 kDa full-length PS2 from transfected COS-7 cells. In 4% paraformaldehyde fixed RAW cells, MA1-754 showed specific Golgi and ER labeling. The MA1-754 immunogen is a synthetic peptide corresponding to residues C L(317) P Y D P E M E E D S Y D S F G E P(334) of human PS2.

Reactivity

Human, Mouse, Rat

Host

Mouse

Isotype

IgG

Antibody clone number

APS 26

Vial size

200 µg

Concentration

1 mg/mL

Storage

-20° C, Avoid Freeze/Thaw Cycles

References

Submitted references

Analysis of presenilin 1 and presenilin 2 expression and processing by newly developed monoclonal antibodies.

Diehlmann A, Ida N, Weggen S, Grünberg J, Haass C, Masters CL, Bayer TA, Beyreuther K
Journal of neuroscience research 1999 May 15;56(4):405-19

---

Analysis of presenilin 1 and presenilin 2 expression and processing by newly developed monoclonal antibodies.

Diehlmann A, Ida N, Weggen S, Grünberg J, Haass C, Masters CL, Bayer TA, Beyreuther K
Journal of neuroscience research 1999 May 15;56(4):405-19

---

Abrogation of the presenilin 1/beta-catenin interaction and preservation of the heterodimeric presenilin 1 complex following caspase activation.

Tesco G, Kim TW, Diehlmann A, Beyreuther K, Tanzi RE
The Journal of biological chemistry 1998 Dec 18;273(51):33909-14

---

Abrogation of the presenilin 1/beta-catenin interaction and preservation of the heterodimeric presenilin 1 complex following caspase activation.

Tesco G, Kim TW, Diehlmann A, Beyreuther K, Tanzi RE
The Journal of biological chemistry 1998 Dec 18;273(51):33909-14

Immunocytochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunofluorescent analysis of Presenilin 2 using Anti-Presenilin 2 Monoclonal Antibody (APS 26) (Product # MA1-754) shows staining in A2058 Cells. Presenilin 2 staining (green), F-Actin staining with Phalloidin (red) and nuclei with DAPI (blue) is shown. Cells were grown on chamber slides and fixed with formaldehyde prior to staining. Cells were probed without (control) or with or an antibody recognizing Presenilin 2 (Product # MA1-754) at a dilution of 1:20 over night at 4°C, washed with PBS and incubated with a DyLight-488 conjugated secondary antibody (Product # 35503, Goat Anti-Mouse). Images were taken at 60X magnification.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunofluorescent analysis of Presenilin 2 using Anti-Presenilin 2 Monoclonal Antibody (APS 26) (Product # MA1-754) shows staining in Hela Cells. Presenilin 2 staining (green), F-Actin staining with Phalloidin (red) and nuclei with DAPI (blue) is shown. Cells were grown on chamber slides and fixed with formaldehyde prior to staining. Cells were probed without (control) or with or an antibody recognizing Presenilin 2 (Product # MA1-754) at a dilution of 1:20 over night at 4°C, washed with PBS and incubated with a DyLight-488 conjugated secondary antibody (Product # 35503, Goat Anti-Mouse). Images were taken at 60X magnification.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunocytochemical staining of PS2 in mouse fibroblasts using Product # MA1-754.