MA1-753

antibody from Invitrogen Antibodies

Targeting: PSEN2 AD3L, AD4, PS2, STM2

Western blot

ELISA

Immunohistochemistry

Antibody data

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Validation data

Product number: MA1-753 - Provider product page
Provider: Invitrogen Antibodies
Product name: PSEN2 Monoclonal Antibody (APS 21)
Antibody type: Monoclonal
Antigen: Synthetic peptide
Description: MA1-753 detects presenilin 2 protein (PS2) from rat, mouse, human, and nonhuman primate samples. No cross-reactivity is seen with presenilin 1. MA1-753 has successfully been used in immuno-fluorescence, Western blot and ELISA procedures. By Western blot, this antibody detects an ~28 kDa protein representing PS2 NT (N-terminus fragment) and the ~46 kDa full-length PS2 from transfected COS-7 cells. In 4% paraformaldehyde fixed COS-7 cells transfected with hPS2, MA1-753 showed specific Golgi, nuclear envelope, and ER labeling. The MA1-753 immunogen is a synthetic peptide corresponding to residues C(31) Q E G R Q G P E D G E N T A(45) of human PS2.
Reactivity: Human, Mouse, Rat
Host: Mouse
Isotype: IgG
Antibody clone number: APS 21
Vial size: 200 µg
Concentration: 1 mg/mL
Storage: -20° C, Avoid Freeze/Thaw Cycles

PSEN2 protein structure - MA1-753 shown in red.

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Immunofluorescent analysis of Presenilin 2 using Anti-Presenilin 2 Monoclonal Antibody (APS 21) (Product # MA1-753) shows staining in A2058 Cells. Presenilin 2 staining (green), F-Actin staining with Phalloidin (red) and nuclei with DAPI (blue) is shown. Cells were grown on chamber slides and fixed with formaldehyde prior to staining. Cells were probed without (control) or with or an antibody recognizing Presenilin 2 (Product # MA1-753) at a dilution of 1:100 over night at 4°C, washed with PBS and incubated with a DyLight-488 conjugated secondary antibody (Product # 35503, Goat Anti-Mouse). Images were taken at 60X magnification.

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Immunofluorescent analysis of Presenilin 2 using Anti-Presenilin 2 Monoclonal Antibody (APS 21) (Product # MA1-753) shows staining in Hela Cells. Presenilin 2 staining (green), F-Actin staining with Phalloidin (red) and nuclei with DAPI (blue) is shown. Cells were grown on chamber slides and fixed with formaldehyde prior to staining. Cells were probed without (control) or with or an antibody recognizing Presenilin 2 (Product # MA1-753) at a dilution of 1:100 over night at 4°C, washed with PBS and incubated with a DyLight-488 conjugated secondary antibody (Product # 35503, Goat Anti-Mouse). Images were taken at 60X magnification.

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Immunofluorescent analysis of Presenilin 2 using Anti-Presenilin 2 Monoclonal Antibody (APS 21) (Product # MA1-753) shows staining in NCI-H460 Cells. Presenilin 2 staining (green), F-Actin staining with Phalloidin (red) and nuclei with DAPI (blue) is shown. Cells were grown on chamber slides and fixed with formaldehyde prior to staining. Cells were probed without (control) or with or an antibody recognizing Presenilin 2 (Product # MA1-753) at a dilution of 1:100 over night at 4°C, washed with PBS and incubated with a DyLight-488 conjugated secondary antibody (Product # 35503, Goat Anti-Mouse). Images were taken at 60X magnification.