H00009530-M02

antibody from Abnova Corporation

Targeting: BAG4 SODD

Western blot (Supportive data in Antibodypedia)

ELISA (Supportive data in Antibodypedia)

Antibody Data

Product number

H00009530-M02

Provider

Abnova Corporation

Proper citation

Abnova Corporation Cat#H00009530-M02, RRID:AB_1203995

Product name

BAG4 monoclonal antibody (M02), clone 2G9

Antibody type

Monoclonal

Description

Mouse monoclonal antibody raised against a full-length recombinant BAG4.

Antigen sequence

MSALRRSGYGPSDGPSYGRYYGPGGGDVPVHPPPP
LYPLRPEPPQPPISWRVRGGGPAETTWLGEGGGGD
GYYPSGGAWPEPGRAGGSHQEQPPYPSYNSNYWNS
TARSRAPYPSTYPVRPELQGQSLNSYTNGAYGPTY
PPGPGANTASYSGAYYAPGYTQTSYSTEVPSTYRS
SGNSPTPVSRWIYPQQDCQTEAPPLRGQVPGYPPS
QNPGMTLPHYPYGDGNRSVPQSGPTVRPQEDAWAS
PGAYGMGGRYPWPSSAPSAPPGNLYMTESTSPWPS
SGSPQSPPSPPVQQPKDSSYPYSQSDQSMNRHNFP
CSVHQYESSGTVNNDDSDLLDSQVQYSAEPQLYGN
ATSDHPNNQDQSSSLPEECVPSDESTPPSIKKIIH
VLEKVQYLEQEVEEFVGKKTDKAYWLLEEMLTKEL
LELDSVETGGQDSVRQARKEAVCKIQAILEKLEKK
GL


Isotype

IgG

Antibody clone number

2G9

Storage

Store at -20°C or lower. Aliquot to avoid repeated freezing and thawing.

Western blot

Supportive validation

Submitted by

Abnova Corporation (provider)

Main image

Experimental details

Western Blot analysis of BAG4 expression in transfected 293T cell line by BAG4 monoclonal antibody (M02), clone 2G9.Lane 1: BAG4 transfected lysate(49.6 KDa).Lane 2: Non-transfected lysate.

ELISA

Supportive validation

Submitted by

Abnova Corporation (provider)

Main image

Experimental details

Detection limit for recombinant GST tagged BAG4 is approximately 3ng/ml as a capture antibody.

Validation comment

Sandwich ELISA (Recombinant protein)

Protocol

Protocol