Antibody data
- Antibody Data
- Antigen structure
- References [1]
- Comments [0]
- Validations
- Flow cytometry [1]
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Validation data
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- Product number
- AF4105 - Provider product page
- Provider
- R&D Systems
- Product name
- Mouse Patched 1/PTCH (First Extracellular Loop) Antibody
- Antibody type
- Polyclonal
- Description
- Antigen Affinity-purified. Detects mouse Patched 1/PTCH in direct ELISAs and Western blots. In direct ELISAs, approximately 50% cross-reactivity with recombinant human Patched 1 is observed, and less than 5% cross-reactivity with recombinant mouse Patched-2 is observed.
- Reactivity
- Mouse
- Host
- Goat
- Conjugate
- Unconjugated
- Antigen sequence
Q61115
- Isotype
- IgG
- Vial size
- 100 ug
- Concentration
- LYOPH
- Storage
- Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Submitted references Toll-like receptor 3 regulates neural stem cell proliferation by modulating the Sonic Hedgehog pathway.
Yaddanapudi K, De Miranda J, Hornig M, Lipkin WI
PloS one 2011;6(10):e26766
PloS one 2011;6(10):e26766
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Supportive validation
- Submitted by
- R&D Systems (provider)
- Main image
- Experimental details
- Detection of Patched 1/PTCH in Neuro-2A Mouse Cell Line by Flow Cytometry. Neuro-2A mouse neuroblastoma cell line was stained with Goat Anti-Mouse Patched 1/PTCH (First Extracellular Loop) Antigen Affinity-purified Polyclonal Antibody (Catalog # AF4105, filled histogram) or isotype control antibody (Catalog # AB-108-C, open histogram), followed by Phycoerythrin-conjugated Anti-Goat IgG Secondary Antibody (Catalog # F0107).