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Western blot
ELISAAntibody data
- Antibody Data
- Antigen structure
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- Validations
- Western blot [1]
- Immunocytochemistry [1]
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- Product number
- R33752 - Provider product page
- Provider
- NSJ Bioreagents
- Product name
- PTCH1 Antibody
- Antibody type
- Polyclonal
- Description
- This highly specific PTCH1 antibody is suitable for use in Western blot/Immunofluorescence/Immunocytochemistry/ELISA applications with human and mouse samples.
- Reactivity
- Human, Mouse
- Host
- Goat
- Conjugate
- Unconjugated
- Vial size
- 100 ug
- Concentration
- 0.5 mg/ml in 1X TBS, pH7.3, with 0.5% BSA (US sourced) and 0.02% sodium azide
- Storage
- Aliquot and store the PTCH1 antibody at -20oC.
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Supportive validation
- Submitted by
- NSJ Bioreagents (provider)
- Main image
- Experimental details
- Western blot testing of mouse fetal kidney lysate with PTCH1 antibody at 2ug/ml. Predicted molecular weight ~161/154/144kDa (isoforms L/M/S) with glycosylated PTCH1 visualized at ~200kDa.
Supportive validation
- Submitted by
- NSJ Bioreagents (provider)
- Main image
- Experimental details
- IF/ICC testing of human HeLa cells with Alexa Fluor 488 secondary (1ug/ml) and PTCH1 antibody (green, 5ug/ml). Blue = DAPI nuclear counterstain.
