R33752

antibody from NSJ Bioreagents

Targeting: PTCH1 BCNS, NBCCS, PTCH

Western blot (Supportive data in Antibodypedia)

ELISA (Recommended by provider)

Immunocytochemistry (Supportive data in Antibodypedia)

Antibody Data

Product number

R33752

Provider

NSJ Bioreagents

Product name

PTCH1 Antibody

Antibody type

Polyclonal

Antigen

Amino acids EELKKINYQVDSWEE were used as the immunogen for this PTCH1 antibody. This sequence is common to the L, M and S isoforms of the protein.

Description

Antigen affinity purified antibody

Reactivity

Human, Mouse

Host

Goat

Conjugate

Unconjugated

Vial size

100 µg

Concentration

0.5 mg/ml

Storage

Aliquot and store the PTCH1 antibody at -20°C.

Western blot

Supportive validation

Submitted by

NSJ Bioreagents (provider)

Main image

Experimental details

Western blot testing of mouse fetal kidney lysate with PTCH1 antibody at 2ug/ml. Predicted molecular weight ~161/154/144kDa (isoforms L/M/S) with glycosylated PTCH1 visualized at ~200kDa.

Immunocytochemistry

Supportive validation

Submitted by

NSJ Bioreagents (provider)

Main image

Experimental details

IF/ICC testing of human HeLa cells with Alexa Fluor 488 secondary (1ug/ml) and PTCH1 antibody (green, 5ug/ml). Blue = DAPI nuclear counterstain.