Antibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Western blot [1]
- Immunocytochemistry [1]
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Validation data
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- Product number
- R33752 - Provider product page
- Provider
- NSJ Bioreagents
- Product name
- PTCH1 Antibody
- Antibody type
- Polyclonal
- Antigen
- Amino acids EELKKINYQVDSWEE were used as the immunogen for this PTCH1 antibody. This sequence is common to the L, M and S isoforms of the protein.
- Description
- Antigen affinity purified antibody
- Reactivity
- Human, Mouse
- Host
- Goat
- Conjugate
- Unconjugated
- Vial size
- 100 µg
- Concentration
- 0.5 mg/ml
- Storage
- Aliquot and store the PTCH1 antibody at -20°C.
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Supportive validation
- Submitted by
- NSJ Bioreagents (provider)
- Main image
- Experimental details
- Western blot testing of mouse fetal kidney lysate with PTCH1 antibody at 2ug/ml. Predicted molecular weight ~161/154/144kDa (isoforms L/M/S) with glycosylated PTCH1 visualized at ~200kDa.
Supportive validation
- Submitted by
- NSJ Bioreagents (provider)
- Main image
- Experimental details
- IF/ICC testing of human HeLa cells with Alexa Fluor 488 secondary (1ug/ml) and PTCH1 antibody (green, 5ug/ml). Blue = DAPI nuclear counterstain.