PA5-87508

antibody from Invitrogen Antibodies

Targeting: PTCH1 BCNS, NBCCS, PTCH

Western blot (Supportive data in Antibodypedia)

Immunocytochemistry (Supportive data in Antibodypedia)

Immunohistochemistry (Supportive data in Antibodypedia)

Other assay (Supportive data in Antibodypedia)

Antibody Data

Product number

PA5-87508

Provider

Invitrogen Antibodies

Product name

PTCH Polyclonal Antibody

Antibody type

Polyclonal

Antigen

Recombinant full-length protein

Description

Immunogen sequence: THSGSDSSDS EYSSQTTVSG LSEELRHYEA QQGAGGPAHQ VIVEATENPV FAHSTVVHPE SRHHPPSNPR QQPHLDSGSL PPGRQGQQPR RDPPREGLW; Positive Samples: 293T, Mouse liver; Cellular Location: Membrane, Multi-pass membrane protein

Reactivity

Human, Mouse, Rat

Host

Rabbit

Isotype

IgG

Vial size

100 µL

Concentration

0.82 mg/mL

Storage

-20° C, Avoid Freeze/Thaw Cycles

References

Submitted references

LncRNA MEG3 restrained pulmonary fibrosis induced by NiO NPs via regulating hedgehog signaling pathway-mediated autophagy.

Gao Q, Chang X, Yang M, Zheng J, Gong X, Liu H, Li K, Wang X, Zhan H, Li S, Feng S, Sun X, Sun Y
Environmental toxicology 2022 Jan;37(1):79-91

Western blot

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Western Blot analysis of PTCH in extracts of various cell lines using PTCH Polyclonal Antibody (Product # PA5-87508) at a dilution of 1:1000. A HRP Goat Anti-Rabbit IgG (H+L) secondary antibody was used at a dilution of 1:10,000. Lysates/proteins: 25 µg per lane. Blocking buffer: 3% nonfat dry milk in TBST.

Immunocytochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunocytochemistry-Immunofluorescence analysis of PTCH was performed in C6 cells using PTCH Polyclonal Antibody (Product # PA5-87508).

Immunohistochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemistry analysis of PTCH in paraffin-embedded Rat ovary using PTCH Polyclonal Antibody (Product # PA5-87508) at a dilution of 1:200.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemistry analysis of PTCH in paraffin-embedded Human tonsil using PTCH Polyclonal Antibody (Product # PA5-87508) at a dilution of 1:200.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemistry analysis of PTCH in paraffin-embedded Human breast using PTCH Polyclonal Antibody (Product # PA5-87508) at a dilution of 1:200.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemistry-Immunofluorescence analysis of PTCH was performed in mouse embryos tissue using PTCH Polyclonal Antibody (Product # PA5-87508) at a dilution of 1:100. Blue: DAPI for nuclear staining.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemistry-Immunofluorescence analysis of PTCH was performed in mouse embryos tissue using PTCH Polyclonal Antibody (Product # PA5-87508) at a dilution of 1:100. Blue: DAPI for nuclear staining.

Other assay

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

3 FIGURE The effects of NiO NPs on Hh pathway and MEG3 in A549 cells. The relative mRNA (A) and protein (B) levels of Shh, Ptch1 Smo, and Gli1 were measured by RT-qPCR ( n = 5) and Western blot ( n = 3), respectively. The expression of MEG3 (C) was detected by RT-qPCR in A549 cells ( n = 5). The data are presented as mean +- SD. * P < .05, versus the control group