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- Antibody Data
- Antigen structure
- References [1]
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- Validations
- Immunocytochemistry [2]
- Immunohistochemistry [3]
- Other assay [1]
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- Product number
- PA5-87508 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- PTCH Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Recombinant full-length protein
- Description
- Immunogen sequence: THSGSDSSDS EYSSQTTVSG LSEELRHYEA QQGAGGPAHQ VIVEATENPV FAHSTVVHPE SRHHPPSNPR QQPHLDSGSL PPGRQGQQPR RDPPREGLW; Positive Samples: 293T, Mouse liver; Cellular Location: Membrane, Multi-pass membrane protein
- Reactivity
- Human, Mouse, Rat
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 100 µL
- Concentration
- 2.79 mg/mL
- Storage
- -20°C, Avoid Freeze/Thaw Cycles
Submitted references LncRNA MEG3 restrained pulmonary fibrosis induced by NiO NPs via regulating hedgehog signaling pathway-mediated autophagy.
Gao Q, Chang X, Yang M, Zheng J, Gong X, Liu H, Li K, Wang X, Zhan H, Li S, Feng S, Sun X, Sun Y
Environmental toxicology 2022 Jan;37(1):79-91
Environmental toxicology 2022 Jan;37(1):79-91
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunocytochemistry-Immunofluorescence analysis of PTCH was performed in C6 cells using PTCH Polyclonal Antibody (Product # PA5-87508).
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunofluorescence analysis of PTCH in mouse embryos cells. Samples were incubated with PTCH Polyclonal antibody (Product # PA5-87508) using a dilution of 1:100. Blue: DAPI for nuclear staining.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemistry analysis of PTCH in paraffin-embedded rat ovary. Samples were incubated with PTCH Polyclonal antibody (Product # PA5-87508) using a dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemistry analysis of PTCH in paraffin-embedded human tonsil. Samples were incubated with PTCH Polyclonal antibody (Product # PA5-87508) using a dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemistry analysis of PTCH in paraffin-embedded human breast. Samples were incubated with PTCH Polyclonal antibody (Product # PA5-87508) using a dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- 3 FIGURE The effects of NiO NPs on Hh pathway and MEG3 in A549 cells. The relative mRNA (A) and protein (B) levels of Shh, Ptch1 Smo, and Gli1 were measured by RT-qPCR ( n = 5) and Western blot ( n = 3), respectively. The expression of MEG3 (C) was detected by RT-qPCR in A549 cells ( n = 5). The data are presented as mean +- SD. * P < .05, versus the control group
