MA1-061

antibody from Invitrogen Antibodies

Targeting: CYTH2 ARNO, CTS18.1, cytohesin-2, PSCD2, PSCD2L, Sec7p-L, Sec7p-like

Immunoprecipitation

Immunohistochemistry

Antibody data

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Validation data

Product number: MA1-061 - Provider product page
Provider: Invitrogen Antibodies
Product name: Cytohesin 2 Monoclonal Antibody (10A12)
Antibody type: Monoclonal
Antigen: Purifed from natural sources
Description: MA1-061 detects recombinant human and mouse cytohesin-2 (ARNO). MA1-061 has been successfully used in IHC (P), immunoprecipitation and IF procedures. By immunoprecipitation this antibody efficiently precipitates GFP-labeled ARNO. MA1-061 does not work in Western blot procedures. The MA1-061 immunogen is purified, His-tagged cytohesin-2.
Reactivity: Human, Mouse
Host: Mouse
Isotype: IgG
Antibody clone number: 10A12
Vial size: 100 µg
Concentration: 1 mg/mL
Storage: -20° C, Avoid Freeze/Thaw Cycles

CYTH2 protein structure - MA1-061 shown in red.

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Immunofluorescent analysis of Cytohesin 2 in HeLa Cells. Cells were grown on chamber slides and fixed with formaldehyde prior to staining. Cells were probed without (control) or with a Cytohesin 2 monoclonal antibody (Product # MA1-061) at a dilution of 1:100 overnight at 4 C, washed with PBS and incubated with a DyLight-488 conjugated secondary antibody (Product # 35503). Cytohesin 2 staining (green), F-Actin staining with Phalloidin (red) and nuclei with DAPI (blue) is shown. Images were taken at 60X magnification.

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Immunofluorescent analysis of Cytohesin 2 in HepG2 Cells. Cells were grown on chamber slides and fixed with formaldehyde prior to staining. Cells were probed without (control) or with a Cytohesin 2 monoclonal antibody (Product # MA1-061) at a dilution of 1:100 overnight at 4 C, washed with PBS and incubated with a DyLight-488 conjugated secondary antibody (Product # 35503). Cytohesin 2 staining (green), F-Actin staining with Phalloidin (red) and nuclei with DAPI (blue) is shown. Images were taken at 60X magnification.

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Immunofluorescent analysis of Cytohesin 2 in NIH-3T3 Cells. Cells were grown on chamber slides and fixed with formaldehyde prior to staining. Cells were probed without (control) or with a Cytohesin 2 monoclonal antibody (Product # MA1-061) at a dilution of 1:100 overnight at 4 C, washed with PBS and incubated with a DyLight-488 conjugated secondary antibody (Product # 35503). Cytohesin 2 staining (green), F-Actin staining with Phalloidin (red) and nuclei with DAPI (blue) is shown. Images were taken at 60X magnification.