SM5009P

antibody from Acris Antibodies GmbH

Targeting: CYTH2 ARNO, CTS18.1, cytohesin-2, PSCD2, PSCD2L, Sec7p-L, Sec7p-like

Western blot

Immunoprecipitation

Antibody data

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Validation data

CYTH2 protein structure - SM5009P shown in red.

Supportive validation

Submitted by: Acris Antibodies GmbH (provider)
Main image
Experimental details: Immunofluorescent analysis of Cytohesin 1,2 in NIH-3T3 Cells. Cells were grown on chamber slides and fixed with formaldehyde prior to staining. Cells were probed without (control) or with a Cytohesin 1,2 monoclonal antibody (Cat.-No SM5009P) at a dilution of 1:100 overnight at 4 C, washed with PBS and incubated with a DyLight-488 conjugated secondary antibody. Cytohesin 1,2 staining (green), F-Actin staining with Phalloidin (red) and nuclei with DAPI (blue) is shown. Images were taken at 60X magnification.

Supportive validation

Submitted by: Acris Antibodies GmbH (provider)
Main image
Experimental details: Immunofluorescent analysis of Cytohesin 1,2 in HepG2 Cells. Cells were grown on chamber slides and fixed with formaldehyde prior to staining. Cells were probed without (control) or with a Cytohesin 1,2 monoclonal antibody (Cat.-No SM5009P) at a dilution of 1:100 overnight at 4 C, washed with PBS and incubated with a DyLight-488 conjugated secondary antibody. Cytohesin 1,2 staining (green), F-Actin staining with Phalloidin (red) and nuclei with DAPI (blue) is shown. Images were taken at 60X magnification.

Supportive validation

Submitted by: Acris Antibodies GmbH (provider)
Main image
Experimental details: Immunofluorescent analysis of Cytohesin 1,2 in NIH-3T3 Cells. Cells were grown on chamber slides and fixed with formaldehyde prior to staining. Cells were probed without (control) or with a Cytohesin 1,2 monoclonal antibody (Cat.-No SM5009P) at a dilution of 1:100 overnight at 4 C, washed with PBS and incubated with a DyLight-488 conjugated secondary antibody. Cytohesin 1,2 staining (green), F-Actin staining with Phalloidin (red) and nuclei with DAPI (blue) is shown. Images were taken at 60X magnification.

Supportive validation

Submitted by: Acris Antibodies GmbH (provider)
Main image
Experimental details: Immunohistochemistry was performed on normal biopsies of deparaffinized human placenta tissue. To expose target proteins, heat induced antigen retrieval was performed using 10mM sodium citrate (pH6.0) buffer, microwaved for 8-15 minutes. Following antigen retrieval tissues were blocked in 3% BSA-PBS for 30 minutes at room temperature. Tissues were then probed at a dilution of 1:500 with a Mouse Monoclonal Antibody recognizing Cytohesin 1,2 (Cat.-No SM5009P) or without primary antibody (negative control) overnight at 4Â°C in a humidified chamber. Tissues were washed extensively with PBST and endogenous peroxidase activity was quenched with a peroxidase suppressor. Detection was performed using a biotin-conjugated secondary antibody and SA-HRP, followed by colorimetric detection using DAB. Tissues were counterstained with hematoxylin and prepped for mounting.

Supportive validation

Submitted by: Acris Antibodies GmbH (provider)
Main image
Experimental details: Immunohistochemistry was performed on normal biopsies of deparaffinized human breast carcinoma. To expose target proteins, heat induced antigen retrieval was performed using 10mM sodium citrate (pH6.0) buffer, microwaved for 8-15 minutes. Following antigen retrieval tissues were blocked in 3% BSA-PBS for 30 minutes at room temperature. Tissues were then probed at a dilution of 1:300 with a Mouse Monoclonal Antibody recognizing Cytohesin 1,2 (Cat.-No SM5009P) or without primary antibody (negative control) overnight at 4Â°C in a humidified chamber. Tissues were washed extensively with PBST and endogenous peroxidase activity was quenched with a peroxidase suppressor. Detection was performed using a biotin-conjugated secondary antibody and SA-HRP, followed by colorimetric detection using DAB. Tissues were counterstained with hematoxylin and prepped for mounting

Supportive validation

Submitted by: Acris Antibodies GmbH (provider)
Main image
Experimental details: Immunofluorescent analysis of Cytohesin 1,2 in HeLa Cells. Cells were grown on chamber slides and fixed with formaldehyde prior to staining. Cells were probed without (control) or with a Cytohesin 1,2 monoclonal antibody (Cat.-No SM5009P) at a dilution of 1:100 overnight at 4 C, washed with PBS and incubated with a DyLight-488 conjugated secondary antibody. Cytohesin 1,2 staining (green), F-Actin staining with Phalloidin (red) and nuclei with DAPI (blue) is shown. Images were taken at 60X magnification.