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Western blotAntibody data
- Antibody Data
- Antigen structure
- References [1]
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- Validations
- Western blot [1]
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- Product number
- 600-401-B06S - Provider product page
- Provider
- Rockland Immunochemicals, Inc.
- Proper citation
- Rockland Cat#600-401-B06S, RRID:AB_1537398
- Product name
- Anti-c-Myb (RABBIT) Antibody - 600-401-B06S
- Antibody type
- Polyclonal
- Vial size
- 25 µl
Submitted references Generating differentially targeted amyloid-beta specific intrabodies as a passive vaccination strategy for Alzheimer's disease.
Sudol KL, Mastrangelo MA, Narrow WC, Frazer ME, Levites YR, Golde TE, Federoff HJ, Bowers WJ
Molecular therapy : the journal of the American Society of Gene Therapy 2009 Dec;17(12):2031-40
Molecular therapy : the journal of the American Society of Gene Therapy 2009 Dec;17(12):2031-40
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Supportive validation
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- Rockland Immunochemicals, Inc. (provider)
- Main image
- Experimental details
- Western Blot of Rabbit anti-C-Myb antibody. Lane 1: Cos7 cell lysates vector. Lane 2: Cos7 cell lysates transfected with c-myb, no treatment. Lane 3: Cos7 cell lysates transfected with c-Myb and heat stress-treated (incubation at 42°C for 30 minutes) to induce c-Myb modification. Load: 35 µg per lane. Primary antibody: cMyb antibody at 1:500 for overnight at 4°C. Secondary antibody: IRDye800 rabbit secondary antibody at 1:10,000 for 45 min at RT. Block: 10% BLOTTO overnight at 4°C. Predicted/Observed size: Lane 3 shows detection of bands at 75, 98, and 125kDa corresponding to overexpressed c-Myb (arrows): wild-type, with one attached SUMO molecule, and with two attached SUMO molecules, respectively.
- Validation comment
- Western Blot
