600-401-C47

antibody from Rockland Immunochemicals, Inc.

Targeting: MYB c-myb

Western blot (Supportive data in Antibodypedia)

Antibody Data

Product number

600-401-C47

Provider

Rockland Immunochemicals, Inc.

Proper citation

Rockland Cat#600-401-C47, RRID:AB_11181306

Product name

Anti-c-Myb pT486 (RABBIT) Antibody - 600-401-C47

Antibody type

Polyclonal

Vial size

100 µl

References

Submitted references

Stress-induced phosphorylation of Thr486 in c-Myb by p38 mitogen-activated protein kinases attenuates conjugation of SUMO-2/3.

Bies J, Sramko M, Wolff L
The Journal of biological chemistry 2013 Dec 27;288(52):36983-93

Western blot

Supportive validation

Submitted by

Rockland Immunochemicals, Inc. (provider)

Main image

Experimental details

Western Blot of Rabbit c-Myb pT486 antibody. Lane 1: Cos7 cells were either null for c-Myb. Lane 2: Cos7 cell lysates transfected with c-myb, no treatment. Lane 3: Cos7 cell lysates transfected with c-Myb andtreated with okadaic acid to incude phosphorylation of c-Myb. Load: 35 µg per lane. Primary antibody: The left blot was probed with anti-c-Myb antibody, the right blot was probed with anti-c-Myb pT486 antibody at 1:2500 for overnight at 4°C. Secondary antibody: IRDye800™ rabbit secondary antibody at 1:10,000 for 45 min at RT. Block: PBS-Tween (0.05%) overnight at 4°C. Predicted/Observed size: 75, 98, and 125kDa corresponding to overexpressed c-Myb (arrow): wild-type, with one attached SUMO molecule, and with two attached SUMO molecules, respectively.

Validation comment

Western Blot