GTX84238

antibody from GeneTex

Targeting: L1CAM CD171, HSAS, HSAS1, MASA, MIC5, S10, SPG1

Western blot (Supportive data in Antibodypedia)

Immunocytochemistry (Supportive data in Antibodypedia)

Immunohistochemistry (Supportive data in Antibodypedia)

Flow cytometry (Supportive data in Antibodypedia)

Antibody Data

Product number

GTX84238

Provider

GeneTex

Proper citation

GeneTex Cat#GTX84238, RRID:AB_10730003

Product name

L1CAM antibody [4F4]

Antibody type

Monoclonal

Reactivity

Human

Host

Mouse

Storage

For short-term storage, store at 4¢XC or aliquot into working amounts and store at -20¢XC. For long-term storage, store at -70¢XC (aliquotted). Avoid repeated freeze-thaw cycles.

Western blot

Supportive validation

Submitted by

GeneTex (provider)

Main image

Experimental details

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY L1CAM (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-L1CAM.

Supportive validation

Submitted by

GeneTex (provider)

Main image

Experimental details

Western blot analysis of extracts (35ug) from 9 different cell lines by using anti-L1CAM monoclonal antibody.

Immunocytochemistry

Supportive validation

Submitted by

GeneTex (provider)

Main image

Experimental details

Anti-L1CAM mouse monoclonal antibody (GTX84238) immunofluorescent staining of COS7 cells transiently transfected with L1CAM

Immunohistochemistry

Supportive validation

Submitted by

GeneTex (provider)

Main image

Experimental details

Immunohistochemical staining of paraffin-embedded Human colon tissue using anti-L1CAM mouse monoclonal antibody. (GTX84238)

Supportive validation

Submitted by

GeneTex (provider)

Main image

Experimental details

Immunohistochemical staining of paraffin-embedded Human endometrium tissue using anti-L1CAM mouse monoclonal antibody. (GTX84238)

Supportive validation

Submitted by

GeneTex (provider)

Main image

Experimental details

Immunohistochemical staining of paraffin-embedded Human Ovary tissue using anti-L1CAM mouse monoclonal antibody. (GTX84238)

Supportive validation

Submitted by

GeneTex (provider)

Main image

Experimental details

Immunohistochemical staining of paraffin-embedded Human prostate tissue using anti-L1CAM mouse monoclonal antibody. (GTX84238)

Supportive validation

Submitted by

GeneTex (provider)

Main image

Experimental details

Immunohistochemical staining of paraffin-embedded Adenocarcinoma of Human colon tissue using anti-L1CAM mouse monoclonal antibody. (GTX84238)

Supportive validation

Submitted by

GeneTex (provider)

Main image

Experimental details

Immunohistochemical staining of paraffin-embedded Adenocarcinoma of Human ovary tissue using anti-L1CAM mouse monoclonal antibody. (GTX84238)

Supportive validation

Submitted by

GeneTex (provider)

Main image

Experimental details

Immunohistochemical staining of paraffin-embedded Human breast tissue using anti-L1CAM mouse monoclonal antibody. (GTX84238)

Flow cytometry

Supportive validation

Submitted by

GeneTex (provider)

Main image

Experimental details

Flow cytometric Analysis of Hela cells, using anti-L1CAM antibody(GTX84238),(Red), compared to a nonspecific negative control antibody(TA50011),(Blue).

Supportive validation

Submitted by

GeneTex (provider)

Main image

Experimental details

HEK293T cells transfected with either L1CAM overexpress plasmid (Red) or empty vector control plasmid (Blue) were immunostained by anti-L1CAM antibody (GTX84238), and then analyzed by flow cytometry.