371606

antibody from BioLegend

Targeting: L1CAM CD171, HSAS, HSAS1, MASA, MIC5, S10, SPG1

Immunohistochemistry (Supportive data in Antibodypedia)

Antibody Data

Product number

371606

Provider

BioLegend

Proper citation

BioLegend Cat#371606, RRID:AB_2632717

Product name

Alexa Fluor® 594 anti-human CD171 (L1CAM)

Antibody type

Monoclonal

Description

CD171, also known as L1CAM or L1, is a transmembrane glycoprotein. It is a 200-220 kD neuronal cell adhesion molecule and a member of the L1 protein family belonging to the immunoglobulin superfamily. This cell adhesion molecule plays an important role in nervous system development, including neuron-neuron adhesion, signal transduction, axon guidance, cell migration and differentiation. CD171 also has a strong implication in treatment-resistanct cancers. Mutations in the gene cause three X-linked neurological syndromes known by the acronym CRASH (corpus callosum hypoplasia, retardation, aphasia, spastic paraplegia and hydrocephalus). Alternative splicing of a neuron-specific exon is thought to be functionally relevant.

Reactivity

Human

Host

Mouse

Conjugate

Yellow dye

Antibody clone number

L1-OV198.5

Vial size

100 µg

Concentration

0.5 mg/ml

Storage

2°C-8°C

Handling

Ambient RT

Immunohistochemistry

Supportive validation

Submitted by

BioLegend (provider)

Main image

Experimental details

Human paraffin-embedded colon tissue slices were prepared with a standard protocol of deparaffination and rehydration. Antigen retrieval was done with Tris-Buffered Saline 20X (1.0M, pH7.4) at 95°C for 40 minutes. Tissue was washed with PBS/ 0.05% Triton X-100 twice for five minutes and blocked with 5% FBS and 0.2% gelatin for 30 minutes. Then, the tissue was stained with 5 µg/ml of anti-human CD171 (L1CAM, clone L1-OV198.5) Alexa Fluor® 594 (red) at 4°C overnight. Nuclei were counterstained with DAPI (blue). The image was captured with a 10X objective.

Conjugate

Yellow dye