13-1719-80

antibody from Invitrogen Antibodies

Targeting: L1CAM CD171, HSAS, HSAS1, MASA, MIC5, S10, SPG1

Antibody data

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Validation data

Product number: 13-1719-80 - Provider product page
Provider: Invitrogen Antibodies
Product name: CD171 Monoclonal Antibody (eBio5G3 (5G3)), Biotin, eBioscience™
Antibody type: Monoclonal
Antigen: Other
Description: Description: The monoclonal antibody eBio5G3 recognizes CD171 also known as neural cell adhesion molecule L1. CD171 is a member of the Ig superfamily containing 6 extracellular Ig domains and five fibronectin type III-like repeats. CD171 has been shown to function as a cell adhesion molecule mediating homotypic and heterotypic cell-cell interactions in neuronal myelination, neurite outgrowth and regeneration. Expression of CD171 has been found on monocytes and mature monocytic-derived and follicular DCs, a minor subset of lymphocytes in addition to that found on neuronal tissue and some tumor cells lines. Expression of CD171 on tumors is thought to contribute to tumor progression. Epitope of eBio5G3 is in amino-terminal Ig-like domain.
Conjugate: Biotin
Antibody clone number: eBio5G3 (5G3)
Concentration: 0.5 mg/mL

L1CAM protein structure - 13-1719-80 shown in red.

Supportive validation

Supportive validation

Supportive validation

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Figure 1 Characterization of neuronal (NDE) and astrocyte derived exosomes (ADE)s. (A) FACS analysis of plasma NDEs and ADEs following the formation of bead-antibody-exosome (BAE)-FITC complexes. Streptavidin magnetic beads were incubated with exosomes isolated from non-psychiatric comparison subjects (NC) and people with schizophrenia (PWS) ( n = 60) and enriched against biotinylated anti-human CD171 biotin (L1CAM, NDE) or anti-GLAST antibody (ADE). BAE complexes are stained with FITC prior to FACS. (B) Plasma NDE and ADE preparations from NC and PWS patients were probed with exosome marker, Flotilin-1 (1:1000). Non-exosome fraction (supernant resulting from 1 h spin at 1500G) served as the negative control while total exosomes (diluted in 1x PBS prior to neuronal and astrocyte enrichment) served as the positive control. The resultant Western Blot demonstrated that NDEs are Flotillin and Neun positive and GFAP negative. However, we were not able to get a signal for the ADEs. Due to multiple freeze-thaw cycles, the integrity of the samples used in the current study had diminished significantly.
Conjugate: Biotin

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Figure 1. Increased number of L1CAM + exosomes in subjects with MDD as compared to controls. (A) Workflow for the isolation, enrichment and analysis of exosomes from human plasma. Magnetic beads were used for enrichment of L1CAM + (brain-enriched) exosomes based on the immunoprecipitation of the L1CAM target bound to the exosomal surface. (B) Expression array validated the accuracy of the exosome isolation and confirmed the exclusion of cellular contamination from cellular vesicles as showed by the low staining for GM130, a marker for cis-Golgi vesicles. In addition to positive (green rectangular) and negative (red rectangular) controls, the expression array detected the following known exosome markers (orange rectangular) in our set of samples: Flot-1, ICAM, Alix, CD81, CD63, EpCAM, ANXA5, TSG101. (C) Western blot analysis confirmed isolation of L1CAM + exosomes. (D) Number of L1CAM + exosomes from subjects in depressive episode during study participation (n=48) as compared to controls (n=23). * indicates significant comparisons with controls. Dashed bars indicate group mean. See also related Figure SI1 .
Conjugate: Biotin

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Figure 7 Neuronally-enriched exosomes (NEEs). ( A ) Schematic depiction of NEE enrichment. ( B ) Fluorescence activated cell sorting (FACS) results of NEEs and negative control (No exosomes). ( C ) Western blot analysis of NEEs, total exosomes (TEs), exosome-depleted serum, and negative controls (No exosomes) with anti-CD171 antibody marker, image cropped was from the same gel. ( D ) Size and concentration analysis of NEEs using Nanoparticle Tracking Analysis System.
Conjugate: Biotin