NBP1-47821

antibody from Novus Biologicals

Targeting: L1CAM CD171, HSAS, HSAS1, MASA, MIC5, S10, SPG1

Western blot (Supportive data in Antibodypedia)

Immunohistochemistry (Supportive data in Antibodypedia)

Flow cytometry (Supportive data in Antibodypedia)

Antibody Data

Product number

NBP1-47821

Provider

Novus Biologicals

Proper citation

Novus Cat#NBP1-47821, RRID:AB_10010800

Product name

Mouse Monoclonal L1CAM Antibody

Antibody type

Monoclonal

Description

Immunogen affinity purified. This antibody is specific for Homo sapiens L1 cell adhesion molecule (L1CAM), transcript variant 1

Reactivity

Human

Host

Mouse

Isotype

IgG

Vial size

0.1 ml

Storage

Store at -20C. Avoid freeze-thaw cycles.

Western blot

Supportive validation

Submitted by

Novus Biologicals (provider)

Main image

Experimental details

Western Blot: L1CAM Antibody (10C12) [NBP1-47821] - HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY L1CAM (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-L1CAM.

Supportive validation

Submitted by

Novus Biologicals (provider)

Main image

Experimental details

Western Blot: L1CAM Antibody (10C12) [NBP1-47821] Analysis of extracts (35ug) from 9 different cell lines by using anti-L1CAM monoclonal antibody.

Immunohistochemistry

Supportive validation

Submitted by

Novus Biologicals (provider)

Main image

Experimental details

Immunohistochemistry-Paraffin: L1CAM Antibody (10C12) [NBP1-47821] - Staining of paraffin-embedded Carcinoma of Human prostate tissue using anti-L1CAM mouse monoclonal antibody.

Supportive validation

Submitted by

Novus Biologicals (provider)

Main image

Experimental details

Immunohistochemistry-Paraffin: L1CAM Antibody (10C12) [NBP1-47821] - Staining of paraffin-embedded Human breast tissue using anti-L1CAM mouse monoclonal antibody.

Supportive validation

Submitted by

Novus Biologicals (provider)

Main image

Experimental details

Immunohistochemistry-Paraffin: L1CAM Antibody (10C12) [NBP1-47821] - Staining of paraffin-embedded Human lung tissue using anti-L1CAM mouse monoclonal antibody.

Supportive validation

Submitted by

Novus Biologicals (provider)

Main image

Experimental details

Immunohistochemistry-Paraffin: L1CAM Antibody (10C12) [NBP1-47821] - Staining of paraffin-embedded Human Ovary tissue using anti-L1CAM mouse monoclonal antibody.

Flow cytometry

Supportive validation

Submitted by

Novus Biologicals (provider)

Main image

Experimental details

Flow Cytometry: L1CAM Antibody (10C12) [NBP1-47821] - HEK293T cells transfected with either overexpression plasmid (Red) or empty vector control plasmid (Blue) were immunostained by anti-L1CAM antibody, and then analyzed by flow cytometry.