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Western blotAntibody data
- Antibody Data
- Antigen structure
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- Validations
- Western blot [1]
- ELISA [1]
- Immunocytochemistry [1]
- Chromatin Immunoprecipitation [2]
- Other assay [1]
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- Product number
- GTX60813 - Provider product page
- Provider
- GeneTex
- Product name
- Histone H2A.Z (acetyl Lys5/Lys7/Lys11) antibody - ChIP grade
- Antibody type
- Polyclonal
- Reactivity
- Human, Mouse
- Host
- Rabbit
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Supportive validation
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- GeneTex (provider)
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- Experimental details
- WB analysis of whole cell extracts (25 £gg, lane 1) from HeLa cells, and 1 £gg of recombinant histone H2A, H2B, H3 and H4 (lane 2, 3, 4 and 5, respectively) using Histone H2A.Z (acetyl Lys5/Lys7/Lys11) antibody at a dilution of 1:1,000 in TBS-Tween containing 5% skimmed milk.
Supportive validation
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- GeneTex (provider)
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- Experimental details
- ELISA was performed using a serial dilution of Histone H2A.Z (acetyl Lys5/Lys7/Lys11) antibody in antigen coated wells. By plotting the absorbance against the antibody dilution, the titer of the antibody was estimated to be 1:56,600.
Supportive validation
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- ICC/IF analysis of HeLa cells using Histone H2A.Z (acetyl Lys5/Lys7/Lys11) antibody (green) and DAPI (blue). Cells were fixed with 4% formaldehyde for 10¡¦ and blocked with PBS/TX-100 containing 5% normal goat serum and 1% BSA. The cells were immunofluorescently labeled with H2A.Zac antibody at a dilution of 1:500.
Supportive validation
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- GeneTex (provider)
- Main image
- Experimental details
- ChIP was performed with HeLa extracts and either control IgG (2 £gg) or Histone H2A.Z (acetyl Lys5/Lys7/Lys11) antibody (1, 2, 5 and 10 £gg per ChIP experiment). The precipitated DNA was detected by qPCR with primers targeting to CCT5 promoter, EIF4A2 promoter, MYT1 gene and the Sat2 satellite repeat.
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- ChIP was performed with K562 extracts and either control IgG (1 £gg) or Histone H2A.Z (acetyl Lys5/Lys7/Lys11) antibody (0.2, 0.5, 1 and 2 £gg per ChIP experiment). The precipitated DNA was detected by qPCR with primers targeting to EIF4A2 promoter, MYT1 gene and the Sat2 satellite repeat.
Supportive validation
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- GeneTex (provider)
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- Experimental details
- Dot blot analysis of peptides containing different acetylations and the unmodified H2A.Z using Histone H2A.Z (acetyl Lys5/Lys7/Lys11) antibody at a dilution of 1:20,000. One hundred to 0.2 pmol of the respective peptides were spotted on a membrane for analysis.
- Validation comment
- Dot
