PA5-114690

antibody from Invitrogen Antibodies

Targeting: H2AZ1 H2A.Z, H2AFZ, H2AZ

Western blot

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Validation data

Product number: PA5-114690 - Provider product page
Provider: Invitrogen Antibodies
Product name: H2A.Zac pan-acetyl (K4,K7,K11,K13) Polyclonal Antibody
Antibody type: Polyclonal
Antigen: Synthetic peptide
Description: Antibody detects endogenous levels of Acetyl-H2A.Z only when acetylated at Lys4/7/11/13.
Reactivity: Human, Mouse, Rat
Host: Rabbit
Isotype: IgG
Vial size: 100 µL
Concentration: 1 mg/mL
Storage: -20°C

H2AZ1 protein structure - PA5-114690 shown in red.

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Immunohistochemistry analysis of H2A.Zac pan-acetyl (K4,K7,K11, K13) in rat spleen tissue. Samples were treated with formaldehyde and treated with citrate buffer for antigen retrieval, blocked, and incubated (1.5 hours at 22°C) with polyclonal antibody (Product # PA5-114690) at a dilution of 1:100. Secondary staining was applied with HRP conjugated anti-Rabbit.

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Immunohistochemistry analysis of H2A.Zac pan-acetyl (K4,K7,K11, K13) in rat brain tissue. Samples were treated with formaldehyde and treated with citrate buffer for antigen retrieval, blocked, and incubated (1.5 hours at 22°C) with polyclonal antibody (Product # PA5-114690) at a dilution of 1:100. Secondary staining was applied with HRP conjugated anti-Rabbit.

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Immunohistochemistry analysis of H2A.Zac pan-acetyl (K4,K7,K11, K13) in rat stomach tissue. Samples were treated with formaldehyde and treated with citrate buffer for antigen retrieval, blocked, and incubated (1.5 hours at 22°C) with polyclonal antibody (Product # PA5-114690) at a dilution of 1:100. Secondary staining was applied with HRP conjugated anti-Rabbit.

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Immunohistochemistry analysis of H2A.Zac pan-acetyl (K4,K7,K11, K13) in human pancreatic cancer. Samples were treated with formaldehyde and treated with citrate buffer for antigen retrieval, blocked, and incubated (1.5 hours at 22°C) with polyclonal antibody (Product # PA5-114690) at a dilution of 1:100. Secondary staining was applied with HRP conjugated anti-Rabbit.

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Immunohistochemistry analysis of H2A.Zac pan-acetyl (K4,K7,K11, K13) in human kidney cancer. Samples were treated with formaldehyde and treated with citrate buffer for antigen retrieval, blocked, and incubated (1.5 hours at 22°C) with polyclonal antibody (Product # PA5-114690) at a dilution of 1:100. Secondary staining was applied with HRP conjugated anti-Rabbit.

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Immunohistochemistry analysis of H2A.Zac pan-acetyl (K4,K7,K11, K13) in human ovarian cancer. Samples were treated with formaldehyde and treated with citrate buffer for antigen retrieval, blocked, and incubated (1.5 hours at 22°C) with polyclonal antibody (Product # PA5-114690) at a dilution of 1:100. Secondary staining was applied with HRP conjugated anti-Rabbit.

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Immunohistochemistry analysis of H2A.Zac pan-acetyl (K4,K7,K11, K13) in mouse lung tissue. Samples were treated with formaldehyde and treated with citrate buffer for antigen retrieval, blocked, and incubated (1.5 hours at 22°C) with polyclonal antibody (Product # PA5-114690) at a dilution of 1:100. Secondary staining was applied with HRP conjugated anti-Rabbit.

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Immunohistochemistry analysis of H2A.Zac pan-acetyl (K4,K7,K11, K13) in mouse kidney tissue. Samples were treated with formaldehyde and treated with citrate buffer for antigen retrieval, blocked, and incubated (1.5 hours at 22°C) with polyclonal antibody (Product # PA5-114690) at a dilution of 1:100. Secondary staining was applied with HRP conjugated anti-Rabbit.

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Immunohistochemistry analysis of H2A.Zac pan-acetyl (K4,K7,K11, K13) in mouse testis tissue. Samples were treated with formaldehyde and treated with citrate buffer for antigen retrieval, blocked, and incubated (1.5 hours at 22°C) with polyclonal antibody (Product # PA5-114690) at a dilution of 1:100. Secondary staining was applied with HRP conjugated anti-Rabbit.

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Immunohistochemistry analysis of H2A.Zac pan-acetyl (K4,K7,K11, K13) in rat liver tissue. Samples were treated with formaldehyde and treated with citrate buffer for antigen retrieval, blocked, and incubated (1.5 hours at 22°C) with polyclonal antibody (Product # PA5-114690) at a dilution of 1:100. Secondary staining was applied with HRP conjugated anti-Rabbit.

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Immunohistochemistry analysis of H2A.Zac pan-acetyl (K4,K7,K11, K13) in human gastric cancer and adjacent normal tissues. Samples were treated with formaldehyde and treated with citrate buffer for antigen retrieval, blocked, and incubated (1.5 hours at 22°C) with polyclonal antibody (Product # PA5-114690) at a dilution of 1:100. Secondary staining was applied with HRP conjugated anti-Rabbit.

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Immunohistochemistry analysis of H2A.Zac pan-acetyl (K4,K7,K11, K13) in mouse muscle tissue. Samples were treated with formaldehyde and treated with citrate buffer for antigen retrieval, blocked, and incubated (1.5 hours at 22°C) with polyclonal antibody (Product # PA5-114690) at a dilution of 1:100. Secondary staining was applied with HRP conjugated anti-Rabbit.

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Immunohistochemistry analysis of H2A.Zac pan-acetyl (K4,K7,K11, K13) in rat heart tissue. Samples were treated with formaldehyde and treated with citrate buffer for antigen retrieval, blocked, and incubated (1.5 hours at 22°C) with polyclonal antibody (Product # PA5-114690) at a dilution of 1:100. Secondary staining was applied with HRP conjugated anti-Rabbit.