PA5-27173

antibody from Invitrogen Antibodies

Targeting: EDNRB ETB, HSCR, HSCR2

Western blot (Supportive data in Antibodypedia)

Immunohistochemistry (Supportive data in Antibodypedia)

Antibody Data

Product number

PA5-27173

Provider

Invitrogen Antibodies

Product name

Endothelin B Receptor Polyclonal Antibody

Antibody type

Polyclonal

Antigen

Synthetic peptide

Description

Recommended positive controls: Mouse brain, Rat brain.

Concentration

1 mg/mL

Western blot

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Western blot analysis of Endothelin B Receptor using 50 µg of mouse brain lysate. Samples were loaded onto a 10% SDS-PAGE gel and probed with an Endothelin B Receptor polyclonal antibody (Product # PA5-27173) at a dilution of 1:3000.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Western blot analysis of Endothelin B Receptor using 50 µg of rat brain lysate. Samples were loaded onto a 10% SDS-PAGE gel and probed with an Endothelin B Receptor polyclonal antibody (Product # PA5-27173) at a dilution of 1:3000.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Western Blot using Endothelin B Receptor Polyclonal Antibody (Product # PA5-27173). Various tissue extracts (50 µg) were separated by 10% SDS-PAGE, and the membrane was blotted with Endothelin B Receptor Polyclonal Antibody (Product # PA5-27173) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.

Immunohistochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Endothelin B Receptor Polyclonal Antibody detects Ednrb protein at membrane on U87 xenograft by immunohistochemical analysis. Sample: Paraffin-embedded U87 xenograft. Endothelin B Receptor Polyclonal Antibody (Product # PA5-27173) dilution: 1:500. Antigen Retrieval: EDTA based buffer, pH 8.0, 15 min.