BAF445

antibody from Novus Biologicals

Targeting: SHH HHG1, HLP3, HPE3, MCOPCB5, SMMCI, TPT, TPTPS

Western blot (Supportive data in Antibodypedia)

Antibody Data

Product number

BAF445

Provider

Novus Biologicals

Product name

Goat Polyclonal Sonic Hedgehog/Shh Antibody

Antibody type

Polyclonal

Description

Antigen Affinity-purified. Detects mouse Sonic Hedgehog/Shh C-Terminus in Western blots. In Western blots, no cross-reactivity with recombinant mouse Shh N-terminal peptide is observed.

Reactivity

Mouse

Host

Goat

Conjugate

Biotin

Isotype

IgG

Vial size

50 ug

Concentration

LYOPH

Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 degreesC as supplied. 1 month, 2 to 8 degreesC under sterile conditions after reconstitution. 6 months, -20 to -70 degreesC under sterile conditions after reconstitution.

Western blot

Supportive validation

Submitted by

Novus Biologicals (provider)

Main image

Experimental details

Detection of Human and Mouse Sonic Hedgehog/Shh by Western Blot. Western blot shows lysates of human stomach tissue and mouse stomach tissue. PVDF membrane was probed with 2 µg/mL of Goat Anti-Mouse Sonic Hedgehog/Shh C-Terminus Biotinylated Antigen Affinity-purified Polyclonal Antibody (Catalog # BAF445) followed by Streptavidin-HRP. A specific band was detected for Sonic Hedgehog/Shh at approximately 50 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.