Antibody data
- Antibody Data
- Antigen structure
- References [6]
- Comments [0]
- Validations
- Western blot [1]
- Immunohistochemistry [1]
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Validation data
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- Product number
- AF445 - Provider product page
- Provider
- R&D Systems
- Product name
- Mouse Sonic Hedgehog/Shh C-Terminus Antibody
- Antibody type
- Polyclonal
- Description
- Immunogen affinity purified. Detects mouse Shh C-terminal peptide in direct ELISAs and Western blots. In these formats, less than 5% cross-reactivity with recombinant mouse (rm) Dhh C-terminal peptide is observed and less than 1% cross-reactivity with a 6X histidine-tagged rmShh N-terminal peptide (amino acids 25-198) is observed. Detects human and mouse Sonic Hedgehog protein in Western blots. Shh C-terminal peptide specific IgG was purified by first passing the goat sera over a mouse Shh N-terminal peptide affinity column. The unbound fraction from the mouse Shh N-terminal peptide affinity column was subsequently purified using a mouse Shh C-terminal peptide affinity column.
- Reactivity
- Mouse
- Host
- Goat
- Conjugate
- Unconjugated
- Antigen sequence
Q62226
- Isotype
- IgG
- Vial size
- 100 ug
- Concentration
- LYOPH
- Storage
- Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Submitted references Loss of the transcription factor GLI1 identifies a signaling network in the tumor microenvironment mediating KRAS oncogene-induced transformation.
T cells enhance stem-like properties and conditional malignancy in gliomas.
GLI1 is regulated through Smoothened-independent mechanisms in neoplastic pancreatic ducts and mediates PDAC cell survival and transformation.
Characterization of progenitor domains in the developing mouse thalamus.
Blockade of hedgehog signaling inhibits pancreatic cancer invasion and metastases: a new paradigm for combination therapy in solid cancers.
Expression of hedgehog proteins in the human thymus.
Mills LD, Zhang Y, Marler RJ, Herreros-Villanueva M, Zhang L, Almada LL, Couch F, Wetmore C, Pasca di Magliano M, Fernandez-Zapico ME
The Journal of biological chemistry 2013 Apr 26;288(17):11786-94
The Journal of biological chemistry 2013 Apr 26;288(17):11786-94
T cells enhance stem-like properties and conditional malignancy in gliomas.
Irvin DK, Jouanneau E, Duvall G, Zhang XX, Zhai Y, Sarayba D, Seksenyan A, Panwar A, Black KL, Wheeler CJ
PloS one 2010 Jun 7;5(6):e10974
PloS one 2010 Jun 7;5(6):e10974
GLI1 is regulated through Smoothened-independent mechanisms in neoplastic pancreatic ducts and mediates PDAC cell survival and transformation.
Nolan-Stevaux O, Lau J, Truitt ML, Chu GC, Hebrok M, Fernández-Zapico ME, Hanahan D
Genes & development 2009 Jan 1;23(1):24-36
Genes & development 2009 Jan 1;23(1):24-36
Characterization of progenitor domains in the developing mouse thalamus.
Vue TY, Aaker J, Taniguchi A, Kazemzadeh C, Skidmore JM, Martin DM, Martin JF, Treier M, Nakagawa Y
The Journal of comparative neurology 2007 Nov 1;505(1):73-91
The Journal of comparative neurology 2007 Nov 1;505(1):73-91
Blockade of hedgehog signaling inhibits pancreatic cancer invasion and metastases: a new paradigm for combination therapy in solid cancers.
Feldmann G, Dhara S, Fendrich V, Bedja D, Beaty R, Mullendore M, Karikari C, Alvarez H, Iacobuzio-Donahue C, Jimeno A, Gabrielson KL, Matsui W, Maitra A
Cancer research 2007 Mar 1;67(5):2187-96
Cancer research 2007 Mar 1;67(5):2187-96
Expression of hedgehog proteins in the human thymus.
Sacedón R, Varas A, Hernández-López C, Gutiérrez-deFrías C, Crompton T, Zapata AG, Vicente A
The journal of histochemistry and cytochemistry : official journal of the Histochemistry Society 2003 Nov;51(11):1557-66
The journal of histochemistry and cytochemistry : official journal of the Histochemistry Society 2003 Nov;51(11):1557-66
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Supportive validation
- Submitted by
- R&D Systems (provider)
- Main image
- Experimental details
- Detection of Human and Mouse Sonic Hedgehog/Shh by Western Blot. Western blot shows lysates of human stomach tissue and mouse stomach tissue. PVDF membrane was probed with 1 µg/mL of Goat Anti-Mouse Sonic Hedgehog/Shh C-Terminus Antigen Affinity-purified Polyclonal Antibody (Catalog # AF445) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF109). A specific band was detected for Sonic Hedgehog/Shh at approximately 50 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
Supportive validation
- Submitted by
- R&D Systems (provider)
- Main image
- Experimental details
- Sonic Hedgehog in Mouse Spinal Cord. Sonic Hedgehog was detected in immersion fixed frozen sections of mouse spinal cord using 15 µg/mL Mouse Sonic Hedgehog C-Terminus Antigen Affinity-purified Polyclonal Antibody (Catalog # AF445) overnight at 4 °C. Tissue was stained with the Anti-Goat HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS008) and counterstained with hematoxylin (blue). View our protocol for Chromogenic IHC Staining of Frozen Tissue Sections.